H19 Overexpression in Breast Adenocarcinoma Stromal Cells Is Associated with Tumor Values and Steroid Receptor Status but Independent of p53 and Ki-67 Expression
| Autor: | Alice Delobelle, Jean-Jacques Curgy, Alain Leprêtre, Séverine Lottin, Fatima Bouali, Domitille Bolle, Jean Coll, Thierry Dugimont, Eric Adriaenssens, Lionel Dumont |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Male Receptors Steroid RNA Untranslated Stromal cell Tumor suppressor gene Muscle Proteins Breast Neoplasms In situ hybridization Adenocarcinoma medicine.disease_cause Breast Neoplasms Male Pathology and Forensic Medicine Gene product Gene expression Biomarkers Tumor medicine Humans Genes Tumor Suppressor Breast In Situ Hybridization biology Middle Aged medicine.disease Gene Expression Regulation Neoplastic Ki-67 Antigen Ki-67 embryonic structures biology.protein Cancer research Female RNA Long Noncoding Stromal Cells Tumor Suppressor Protein p53 Carcinogenesis Regular Articles |
| Zdroj: | The American Journal of Pathology. 153:1597-1607 |
| ISSN: | 0002-9440 |
| DOI: | 10.1016/s0002-9440(10)65748-3 |
| Popis: | In a previous study we described the expression of the H19 gene by in situ hybridization (ISH) in normal breast and in benign or malignant breast tumors (Dugimont T, Curgy JJ, Wernert N, Delobelle A. Raes MB, Joubel A, Stehelin D, Coll J: Biol Cell 1995, 85:117–124). In the present work, 1) we extend the previous one to a statistically useful number of adenocarcinomas, including 10 subclasses, 2) we provide information on the precise ISH localization of the H19 RNA by using, on serial tissue sections, antibodies delineating specifically the stromal or the epithelial component of the breast, and 3) we consider relationships between the H19 gene expression and various clinicopathological information as tumor values (T0 to T4), grades, steroid receptors, lymph node status, and molecular features as the p53 gene product and the Ki-67/MIB-1 protein, which is specific to proliferating cells. Data indicate that 1) in 72.5% of studied breast adenocarcinomas an overall H19 gene expression is increased when compared with healthy tissues, 2) the H19 gene is generally overexpressed in stromal cells (92.2%) and rarely in epithelial cells (2.9% only), 3) an up-regulation of the H19 gene is significantly correlated with the tumor values and the presence of both estrogen and progesterone receptors, and 4) at the cellular level, the H19 gene demonstrates an independent expression versus accumulation of both the p53 protein and the Ki-67/MIB-1 cell-cycle marker. |
| Databáze: | OpenAIRE |
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