ExciTides: NTP-derived probes for monitoring pyrophosphatase activity based on excimer-to-monomer transitions
| Autor: | Joanna Kowalska, Jacek Jemielity, Renata Kasprzyk, Michal Kopcial, Dominika Strzelecka, Przemyslaw Wanat, Pawel J. Sikorski |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
biology 010405 organic chemistry Chemistry Metals and Alloys Pyrophosphatase activity General Chemistry 010402 general chemistry Excimer 01 natural sciences Fluorescence Combinatorial chemistry Catalysis Cycloaddition Enzyme assay 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry.chemical_compound Monomer Materials Chemistry Ceramics and Composites Click chemistry biology.protein Nucleotide |
| Zdroj: | Chemical Communications. 54:9773-9776 |
| ISSN: | 1364-548X 1359-7345 |
| DOI: | 10.1039/c8cc04968h |
| Popis: | We describe a new type of nucleotide-derived fluorescent probe designed for monitoring pyrophosphatase activity based on excimer-to-monomer transitions, called ExciTide. The nucleotides were designed with two self-interacting dye moieties and synthesised using copper-catalysed azide-alkyne cycloaddition click chemistry. We applied these probes for enzyme activity monitoring and inhibitor evaluation. Some of the probes permeated into living cells, yielding interesting prospects for future applications. |
| Databáze: | OpenAIRE |
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