Neuroprotective Activity of Polyphenol-Rich Ribes diacanthum Pall against Oxidative Stress in Glutamate-Stimulated HT-22 Cells and a Scopolamine-Induced Amnesia Animal Model
| Autor: | Seung Yeon Baek, Dai-Eun Sok, Young-Jun Kim, Mee Ree Kim, Kun Jong Lee, Hyun Jeong Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Antioxidant
Physiology medicine.medical_treatment Clinical Biochemistry Flavonoid Pharmacology medicine.disease_cause Biochemistry Neuroprotection Article 03 medical and health sciences 0302 clinical medicine BDNF/TrkB pathway Ribes diacanthum Pall medicine Viability assay Akt/Nrf2/ARE pathway Molecular Biology chemistry.chemical_classification Chemistry lcsh:RM1-950 Glutamate receptor Cell Biology In vitro neuroprotective effect lcsh:Therapeutics. Pharmacology Apoptosis 030220 oncology & carcinogenesis 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Antioxidants Volume 9 Issue 9 Antioxidants, Vol 9, Iss 895, p 895 (2020) |
| ISSN: | 2076-3921 |
| DOI: | 10.3390/antiox9090895 |
| Popis: | Ribes diacanthum Pall, a native Mongolian medicinal plant, has been reported to show antioxidant activities due to its polyphenol and flavonoid content, and is especially rich in the ethyl acetate fraction from an 80% methanol extraction (RDP). We assessed the cytoprotective effect of RDP on glutamate-caused oxidative stress and apoptosis in mouse hippocampal neuronal cells (HT-22 cells). Cell viability was significantly recovered by RDP treatment. Also, RDP effectively decreased the glutamate-induced production of intracellular reactive oxygen species (ROS). In flow cytometric analysis, apoptotic cells and the mitochondrial membrane potential were suppressed by RDP. In the Western blotting analysis, we found that RDP not only decreased the release of apoptotic proteins but also recovered anti-apoptotic protein. Additionally, RDP enhanced the antioxidant defense system by regulating the expression of antioxidant enzymes. Furthermore, treatment with RDP activated the BDNF/TrkB pathway. In accordance with the in vitro results, RDP meliorated memory deficit by defending hippocampal neuronal cells against oxidative damage in scopolamine-injected mice. Taken together, our present study showed that RDP exerted antioxidant and neuroprotective actions against oxidative stress. Therefore, RDP might facilitate the development of candidates for functional health foods for neurodegenerative disorders. |
| Databáze: | OpenAIRE |
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