Promyelocytic leukemia-nuclear body formation is an early event leading to retinoic acid-induced differentiation of neuroblastoma cells

Autor: Camille Ripoll, Fahd Benjelloun, A. Delaune, E. Legrand, Cécile Corbière, Vannier Jp, Marc Vasse
Přispěvatelé: Université de Caen Normandie (UNICAEN), Normandie Université (NU), Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur (RIIP), Université de Rouen Normandie (UNIROUEN)
Rok vydání: 2007
Předmět:
MESH: Neoplasm Proteins
MESH: Signal Transduction
MAPK/ERK pathway
[SDV]Life Sciences [q-bio]
viruses
Cellular differentiation
Retinoic acid
Promyelocytic Leukemia Protein
Biochemistry
Neuroblastoma
chemistry.chemical_compound
0302 clinical medicine
MESH: Promyelocytic Leukemia Protein
Enzyme Inhibitors
Phosphorylation
Nuclear protein
Extracellular Signal-Regulated MAP Kinases
MESH: Extracellular Signal-Regulated MAP Kinases
0303 health sciences
Nuclear Proteins
virus diseases
Cell Differentiation
MESH: Transcription Factors
MESH: Gene Expression Regulation
Neoplastic
Neoplasm Proteins
3. Good health
Gene Expression Regulation
Neoplastic
MESH: Enzyme Inhibitors
030220 oncology & carcinogenesis
[SDE]Environmental Sciences
Signal transduction
Signal Transduction
medicine.drug
MESH: Cell Differentiation
medicine.medical_specialty
MESH: Cell Line
Tumor
education
Tretinoin
Biology
Models
Biological
03 medical and health sciences
Cellular and Molecular Neuroscience
Promyelocytic leukemia protein
Keratolytic Agents
MESH: Keratolytic Agents
Cell Line
Tumor
MESH: Cell Proliferation
Internal medicine
medicine
Humans
MESH: Tumor Suppressor Proteins
MESH: Cell Nucleus Structures
Cell Proliferation
030304 developmental biology
MESH: Tretinoin
MESH: Humans
MESH: Phosphorylation
Tumor Suppressor Proteins
MESH: Models
Biological
medicine.disease
MESH: Neuroblastoma
Cell Nucleus Structures
Endocrinology
chemistry
Cancer research
biology.protein
MESH: Nuclear Proteins
Transcription Factors
Zdroj: Journal of Neurochemistry
Journal of Neurochemistry, Wiley, 2007, 104 (1), pp.071106220615005-???. ⟨10.1111/j.1471-4159.2007.05019.x⟩
ISSN: 1471-4159
0022-3042
DOI: 10.1111/j.1471-4159.2007.05019.x
Popis: International audience; Neuroblastoma is one of the most common cancers in children. Neuroblastoma differentiation is linked to the presence of the promyelocytic leukemia (PML) protein. Retinoic acid, a powerful differentiation-inducer in vitro, is a potent agent for the treatment of neuroblastoma. Using two different human neuroblastoma cell lines, SH-SY5Y and LA-N-5, we show here that PML protein leads to the formation of nuclear bodies (PML-NB) after only 1 h of retinoic acid treatment and that this formation is mediated by the extracellular signal-regulated kinase (ERK) pathway. Inhibition of protein kinase C also leads to formation of PML-NB via the ERK pathway. Both sumoylation and phosphorylation of PML in an ERK-dependent pathway are also required for formation of PML-NB. Finally, we show that PML-NB formation in neuroblastoma cells is associated with neurite outgrowth. These results support the proposal that the formation of PML-NB is correlated with the differentiation of neuroblastoma cells.
Databáze: OpenAIRE
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