D-mef2 is a target for Tinman activation during Drosophila heart development
| Autor: | Young Mi Lee, Eric N. Olson, Yongsok Kim, Robert A. Schulz, Kathleen M Gajewski |
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| Rok vydání: | 1997 |
| Předmět: |
Mef2
animal structures Transcription Genetic Recombinant Fusion Proteins Molecular Sequence Data Biology General Biochemistry Genetics and Molecular Biology Animals Drosophila Proteins Enhancer Heart formation Molecular Biology Transcription factor Homeodomain Proteins Genetics Binding Sites Base Sequence General Immunology and Microbiology Heart development MEF2 Transcription Factors General Neuroscience Gene Expression Regulation Developmental Cell Differentiation Heart Immunohistochemistry Upstream Enhancer DNA-Binding Proteins Repressor Proteins Drosophila melanogaster Enhancer Elements Genetic Myogenic Regulatory Factors embryonic structures Trans-Activators Homeobox Sequence Analysis Drosophila Protein Transcription Factors Research Article |
| Zdroj: | The EMBO Journal. 16:515-522 |
| ISSN: | 1460-2075 |
| DOI: | 10.1093/emboj/16.3.515 |
| Popis: | The NK-type homeobox gene tinman and the MADS box gene D-mef2 encode transcription factors required for the development and differentiation of the Drosophila heart, and closely related genes regulate cardiogenesis in vertebrates. Genetic analyses indicate that tinman and D-mef2 act at early and late steps, respectively, in the cardiogenic lineage. However, it is unknown whether regulatory interactions exist between these developmental control genes. We show that D-mef2 expression in the developing Drosophila heart requires a novel upstream enhancer containing two Tinman binding sites, both of which are essential for enhancer function in cardiac muscle cells. Transcriptional activity of this cardiac enhancer is dependent on tinman function, and ectopic Tinman expression activates the enhancer outside the cardiac lineage. These results define the only known in vivo target for transcriptional activation by Tinman and demonstrate that D-mef2 lies directly downstream of tinman in the genetic cascade controlling heart formation in Drosophila. |
| Databáze: | OpenAIRE |
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