The effect of voluntary wheel running on the antioxidant status is dependent on sociability conditions

Autor: Roberta de Paula Martins, Mauricio P. Cunha, Daniele G. Machado, Andreza Fabro de Bem, Alcir Luiz Dafre, Alexandra Latini, Gianni Mancini, Ana Lúcia S. Rodrigues, Viviane Glaser
Jazyk: angličtina
Rok vydání: 2020
Předmět:
GSSG
Oxidized glutathione
Male
Antioxidant
Ethylenediamine tetraacetic acid (EDTA)
Ethylene glycol-bis(β-aminoethyl ether)-N
N
N′
N′-tetraacetic acid (also known as Egtazic acid
EGTA)
Thiobarbituric acid
medicine.medical_treatment
Clinical Biochemistry
Glutathione reductase
Toxicology
medicine.disease_cause
Biochemistry
TST
Tail suspension test
Antioxidants
Behavioral Neuroscience
chemistry.chemical_compound
Mice
0302 clinical medicine
SIS
Social isolation stress
Social isolation stress
Cerebellum
GR
Glutathione reductase
chemistry.chemical_classification
Cerebral Cortex
Behavior
Animal
Chemistry
Glutathione peroxidase
Voluntary wheel running
TBARS
Thiobarbituric acid reactive species
DTNB
5
5-dithiobis-2-nitrobenzoic acid
Glutathione
Housing
Animal
Mitochondria
medicine.anatomical_structure
Glutathione Reductase
Liver
Social Isolation
Cerebral cortex
GPx
Glutathione peroxidase
NADPH
Nicotinamide adenine dinucleotide phosphate
ANOVA
Analysis of variance
NADH
Nicotinamide adenine dinucleotide
CHP
Cumene peroxide
medicine.medical_specialty
PA
Physical activity
Motor Activity
Thiobarbituric Acid Reactive Substances
Article
GSH
Reduced glutathione
03 medical and health sciences
Internal medicine
Physical Conditioning
Animal
medicine
TBARS
Animals
Sulfhydryl Compounds
Muscle
Skeletal
MDA
Malondialdehyde
Biological Psychiatry
Pharmacology
Glutathione Peroxidase
Physical activity
Myocardium
030227 psychiatry
Oxidative Stress
Endocrinology
NPSH
Nonprotein thiols groups
Lipid Peroxidation
030217 neurology & neurosurgery
Oxidative stress
Stress
Psychological
Zdroj: Pharmacology, Biochemistry, and Behavior
ISSN: 1873-5177
0091-3057
Popis: Voluntary wheel running is widely used as a physical activity (PA) model in rodents, but most studies investigate the beneficial effects of this intervention in socially isolated mice. Social isolation stress (SIS) is associated with vulnerability to oxidative stress and reduced mitochondrial activity. Thus, the aim of this study was to investigate the effects of free access to a running wheel for 21 days on the various markers of the cellular redox/antioxidant status as well as mitochondrial function of mice subjected to SIS or maintained in groups of 3 in the homecage. SIS increased thiobarbituric acid reactive substance (TBARS) levels in the cerebral cortex, and PA intervention was not able to reverse such alteration. PA reduced TBARS levels in the liver of grouped mice and gastrocnemius of socially isolated mice. PA increased nonprotein thiol (NPSH) levels in the cerebral cortex of grouped mice. Furthermore, socially isolated mice presented lower glutathione peroxidase (GPx) activity in the cerebellum and gastrocnemius, and glutathione reductase (GR) activity in the cerebral cortex and liver. By contrast, SIS induced higher GPx activity in the cerebral cortex and heart. PA reduced GPx (cerebral cortex) and GR (cerebral cortex and liver) activities of socially isolated mice. SIS caused higher activity of mitochondrial complexes I and II in the cerebral cortex, and the PA paradigm was not able to alter this effect. Interestingly, the PA produced antidepressant-like effect at both SIS and control groups. In conclusion, the results showed the influence of SIS for the effects of PA on the antioxidant status, but not on the mitochondrial function and emotionality.
Highlights • PA intervention produces antioxidant responses dependent on sociability conditions. • SIS induces mitochondria function and antioxidant defense abnormalities. • Running produces antidepressant-like behavior and does not change the ambulation. • The distance travelled on the running wheel is correlated with immobility time in the TST. • The lipoperoxidation index is negatively correlated with time spent on the running wheel.
Databáze: OpenAIRE
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