Preparation and purification of ?? enolase (neuron-specific enolase) using high performance anion exchange chromatography
| Autor: | M.R. Ven Murthy, B. Dastugue, J L Viallard |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Brain Chemistry
Chromatography Ion exchange Tissue Extracts Chemistry Ion chromatography Enolase Fraction (chemistry) General Medicine Chromatography Ion Exchange Biochemistry High-performance liquid chromatography Rats Isoenzymes Gel permeation chromatography Cellular and Molecular Neuroscience Phosphopyruvate Hydratase Yield (chemistry) Animals Humans Cattle Electrophoresis Polyacrylamide Gel Polyacrylamide gel electrophoresis Chromatography High Pressure Liquid |
| Zdroj: | Neurochemical Research. 13:31-35 |
| ISSN: | 1573-6903 0364-3190 |
| DOI: | 10.1007/bf00971851 |
| Popis: | A simple and rapid method, using only two chromatographic steps, is described for the purification and preparation of gamma gamma enolase isoenzymes from human and beef brain extracts. In the first step, a crude gamma gamma enolase was obtained by chromatography on Q-Sepharose Fast Flow column. The crude fraction was then purified by high performance anion exchange chromatography on a Mono-Q column. gamma gamma enolase obtained in this manner was shown to be homogeneous by two dimensional polyacrylamide gel electrophoresis and by high performance gel permeation chromatography. The yield of gamma gamma enolase by this method was 7-8 mg of pure enzyme per 100 g of brain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink