Cell wall modification and lignin biosynthesis involved in disease resistance against Diaporthe citri in harvested pummelo fruit elicited by carvacrol
| Autor: | Nan Cai, Qiang Huang, Chunpeng Wan, Jinyin Chen, Chuying Chen |
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| Rok vydání: | 2021 |
| Předmět: |
Citrus
food.ingredient Pectin Lignin Cell wall chemistry.chemical_compound food Ascomycota Cell Wall Pectinase Cell wall modification Disease Resistance Nutrition and Dietetics biology food and beverages Diaporthe citri biology.organism_classification Horticulture Phomopsis chemistry Fruit Postharvest Cymenes Agronomy and Crop Science Food Science Biotechnology |
| Zdroj: | Journal of the Science of Food and Agriculture. 102:3140-3149 |
| ISSN: | 1097-0010 0022-5142 |
| Popis: | Background Phomopsis stem-end rot caused by Diaporthe citri, causes significant commercial postharvest losses of pummelo fruit during storage. Carvacrol (CVR) is a known generally recognized as safe (GRAS) and has the ability to prolong the preservation of harvested fruits. In the present study, the inhibitory effects of CVR treatment at the appropriate concentration on Phomopsis stem-end rot development of harvested pummelo fruit inoculated with D. citri were evaluated by the amounts of cell wall components, the activities and gene expressions of related enzymes involved in cell wall modification and lignin biosynthesis. Results Results indicated that CVR completely inhibited D. citri growth in vitro at 200 mg L-1 and significantly controlled Phomopsis stem-end rot development in harvested pummelo. The CVR treatment delayed peel softening and browning, and retarded electrolyte leakage, O2 •- production, and malondialdehyde content. The CVR-treated fruit maintained higher amounts of cell wall materials (CWM), protopectin, hemicelluloses, and cellulose, but exhibited lower water-soluble pectin (WSP) amount. Moreover, in D. citri-inoculated fruit, CVR treatment suppressed the activities and gene expressions of cell wall disassembling-enzymes, including pectin methylesterase, polygalacturonase, cellulase, and β-galactosidase, while the development of cell wall degradation was reduced. Meanwhile, the CVR treatment enhanced the lignin biosynthesis by increasing the activities and up-regulating the gene expressions of phenylalanine ammonialyase, cinnamic alcohol dehydrogenase, and peroxidase accompanied with elevated level of lignin in pummelo fruit. Conclusion The disease resistance to D. citri in pummelo fruit elicited by CVR treatment is related to delaying cell wall degradation and enhancing lignin biosynthesis. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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