Estradiol deficiency and skeletal muscle apoptosis: Possible contribution of microRNAs
| Autor: | Dawn A. Lowe, Sira Karvinen, Christine A. Cabelka, Hanna Kaarina Juppi, Eija K. Laakkonen, Gengyun Le, Tara L. Mader |
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| Rok vydání: | 2020 |
| Předmět: |
estrogeenit
0301 basic medicine estradioli Aging medicine.medical_specialty vaihdevuodet caspase menopause Apoptosis Biochemistry Article hormonaaliset tekijät 03 medical and health sciences Mice cytochrome C 0302 clinical medicine Endocrinology Western blot Internal medicine microRNA Genetics medicine Animals Muscle Skeletal Molecular Biology Caspase Estrous cycle Messenger RNA medicine.diagnostic_test biology Estradiol sytokromit RNA Skeletal muscle Cell Biology Mice Inbred C57BL MicroRNAs ovariectomy 030104 developmental biology medicine.anatomical_structure muscle mass lihasmassa biology.protein Female mikro-RNA hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery |
| Zdroj: | Exp Gerontol |
| ISSN: | 1873-6815 |
| Popis: | Background Menopause leads to estradiol (E2) deficiency that is associated with decreases in muscle mass and strength. Here we studied the effect of E2 deficiency on miR-signaling that targets apoptotic pathways. Methods C57BL6 mice were divided into control (normal estrous cycle, n = 8), OVX (E2 deficiency, n = 7) and OVX + E2 groups (E2-pellet, n = 4). Six weeks following the OVX surgery, mice were sacrificed and RNA isolated from gastrocnemius muscles. miR-profiles were studied with Next-generation sequencing (NGS) and candidate miRs verified using qPCR. The target proteins of the miRs were found using in silico analysis and measured at mRNA (qPCR) and protein levels (Western blot). Results Of the apoptosis-linked miRs present, eleven (miRs-92a-3p, 122-5p, 133a-3p, 214-3p, 337-3p, 381-3p, 483-3p, 483-5p, 491-5p, 501-5p and 652-3p) indicated differential expression between OVX and OVX + E2 mice in NGS analysis. In qPCR verification, muscle from OVX mice had lower expression of all eleven miRs compared with OVX + E2 (p |
| Databáze: | OpenAIRE |
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