Influence of15N enrichment on the net isotopic fractionation factor during the reduction of nitrate to nitrous oxide in soil

Autor: Francis Andreux, Per Ambus, Catherine Hénault, Jean Lévêque, Olivier Mathieu, Marie-Jeanne Milloux
Přispěvatelé: Microbiologie du Sol et de l'Environnement (MSE), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Biosystems Division [Roskilde], Risø National Laboratory for Sustainable Energy (Risø DTU), Technical University of Denmark [Lyngby] (DTU)-Technical University of Denmark [Lyngby] (DTU), Work funded by the Conseil Régional de Bourgogne, Microbiologie du Sol et de l'Environnement ( MSE ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ), Risø National Laboratory for Sustainable Energy ( Risø DTU ), Technical University of Denmark [Lyngby] ( DTU ) -Technical University of Denmark [Lyngby] ( DTU )
Rok vydání: 2007
Předmět:
Denitrification
Abundance (chemistry)
[SDE.MCG]Environmental Sciences/Global Changes
Nitrous Oxide
chemistry.chemical_element
Fractionation
Chemical Fractionation
Gas Chromatography-Mass Spectrometry
Analytical Chemistry
Soil
chemistry.chemical_compound
Nitrate
[SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry
[ SDU.ENVI ] Sciences of the Universe [physics]/Continental interfaces
environment
Soil Pollutants
[SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces
environment
Spectroscopy
Nitrates
Nitrogen Isotopes
Chemistry
Organic Chemistry
Substrate (chemistry)
Nitrous oxide
[ SDU.STU.GC ] Sciences of the Universe [physics]/Earth Sciences/Geochemistry
Nitrogen
[ SDE.MCG ] Environmental Sciences/Global Changes
Environmental chemistry
Soil water
Oxidation-Reduction
Zdroj: Rapid Communications in Mass Spectrometry
Rapid Communications in Mass Spectrometry, Wiley, 2007, 21 (8), pp.1447-1451. ⟨10.1002/rcm.2979⟩
Rapid Communications in Mass Spectrometry, Wiley, 2007, 21 (8), pp.1447-1451. 〈10.1002/rcm.2979〉
ISSN: 1097-0231
0951-4198
DOI: 10.1002/rcm.2979
Popis: 5 pages; International audience; Nitrous oxide, a greenhouse gas, is mainly emitted from soils during the denitrification process. Nitrogen stable-isotope investigations can help to characterise the N(2)O source and N(2)O production mechanisms. The stable-isotope approach is increasingly used with (15)N natural abundance or relatively low (15)N enrichment levels and requires a good knowledge of the isotopic fractionation effect inherent to this biological mechanism. This paper reports the measurement of the net and instantaneous isotopic fractionation factor (alpha(s/p) (i)) during the denitrification of NO(3) (-) to N(2)O over a range of (15)N substrate enrichments (0.37 to 1.00 atom% (15)N). At natural abundance level, the isotopic fractionation effect reported falls well within the range of data previously observed. For (15)N-enriched substrate, the value of alpha(s/p) (i) was not constant and decreased from 1.024 to 1.013, as a direct function of the isotopic enrichment of the labelled nitrate added. However, for enrichment greater than 0.6 atom% (15)N, the value of alpha(s/p) (i) seems to be independent of substrate isotopic enrichment. These results suggest that for isotopic experiments applied to N(2)O emissions, the use of low (15)N-enriched tracers around 1.00 atom% (15)N is valid. At this enrichment level, the isotopic effect appears negligible in comparison with the enrichment of the substrate.
Databáze: OpenAIRE
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