Uroven' visfatina syvorotki krovi i ekspressiyagena visfatina (PBEF1) v podkozhnoyi vistseral'noy zhirovoy tkani u detey
| Autor: | Dedov I I, Sosunov, Peterkova V A, Kosygina A |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Physiology Endocrinology Diabetes and Metabolism Adipose tissue QD415-436 Overweight Age and sex Biochemistry Anthropometric parameters Normal body weight Endocrinology children Internal medicine visfatin Internal Medicine medicine QP1-981 adolescents Nutrition and Dietetics business.industry Public Health Environmental and Occupational Health Anthropometry Immunoenzyme assay adipose tissue Mrna level gene expression medicine.symptom business |
| Zdroj: | Ожирение и метаболизм, Vol 7, Iss 4, Pp 20-23 (2010) |
| ISSN: | 2306-5524 2071-8713 |
| Popis: | The aim of this study was to investigate the characteristics of visfatin - PBEF1 - gene expression in subcutaneous and visceral adipose tissue and serum levels of visfatin in children with relation to age and anthropometric parameters. The study included 52 patients (27 boys (52%), 25 girls (48%) aged from 2,5 to 18 years (13,8 [7,4-15,1] years)), who underwent an elective surgical intervention. PBEF1 mRNA level was measured by real-time PCR and serum level of visfatin was quantified by immunoenzyme assay. According to our study visfatin serum concentration in children with normal body weight was 9,2 [7,4-11,6] ng/ml, whereas in the overweight group - 9,5 [7,5-11,1] ng/ml (p=0,5). No statistically significant gender difference in serum visfatin levels was observed. No correlation between visfatin levels and age, pubertal stages and anthropometric indices in children was found. Statistically significant differences in the level of gene expression between subcutaneous and visceral adipose tissue were found (р=0,002) in the total group of children surveyed and children with normal weight, while there were no depot-specific differences in overweight children. The study did not reveal any dependence of PBEF1 expression on age and sex of children. Expression of PBEF1 in adipose tissue decreases with puberty (PBEF1VATγ=-0,24, р=0,02; PBEF1SATγ=-0,25, р=0,02). PBEF1 expression in adipose tissue was not correlated with the serum visfatin (R=-0,06, р=0,6). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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