Gallium enhances reconstructive properties of a calcium phosphate bone biomaterial

Autor: Jean-Michel Bouler, Ivana Strazic Geljic, Pascal Janvier, Jean-Claude Scimeca, Nicolas Melis, Florian Boukhechba, Charlotte Mellier, Sébastien Schaub, Elise Verron, Jean-Pierre Laugier
Přispěvatelé: Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), GRAFTYS SA [Aix en Provence, France], Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Centre Commun de Microscopie Appliquée [Nice] (CCMA), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Nice University, CNRS, Graftys SA (Aix‐en‐Provence, France) and INSERM supported this work.This work was sponsored by Graftys SA, Convention CIFRE N°2011/1356., Jehan, Frederic, Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Université de Nantes (UN)-Université de Nantes (UN)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Graftys SARL, Institute of Developmental Biology and Cancer (IBDC), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Université de Nantes - Faculté des Sciences et des Techniques, Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS), Centre Commun de Microscopie Appliquée (CCMA), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)
Rok vydání: 2017
Předmět:
Calcium Phosphates
0301 basic medicine
Medicine (miscellaneous)
bone reconstruction
bone repair quantification
calcium phosphate cements
gallium
gallium bone delivery
osteoclast/osteoblast crosstalk
second harmonic generation quantitative analysis
Biocompatible Materials
Gallium
Bone tissue
Bone remodeling
0302 clinical medicine
Apatites
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
Bone cell
Femur
Cells
Cultured
[SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system
[SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology
Chemistry
[SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology
Bone Cements
Biomaterial
Cell Differentiation
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biomolecules [q-bio.BM]
3. Good health
medicine.anatomical_structure
[SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system
030220 oncology & carcinogenesis
Biomedical Engineering
[SDV.CAN]Life Sciences [q-bio]/Cancer
Bone healing
Bone resorption
Osseointegration
Biomaterials
03 medical and health sciences
Osteoclast
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
medicine
Animals
Humans
[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials
Cell Shape
Cell Proliferation
Osteoblasts
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
Rats
030104 developmental biology
Bone Substitutes
Biomedical engineering
Zdroj: Journal of Tissue Engineering and Regenerative Medicine
Journal of Tissue Engineering and Regenerative Medicine, John Wiley & Sons Ltd., 2017, pp.854-866. ⟨10.1002/term.2396⟩
Journal of Tissue Engineering and Regenerative Medicine, 2017, 12, pp.854-866. ⟨10.1002/term.2396⟩
Journal of Tissue Engineering and Regenerative Medicine, John Wiley & Sons Ltd., 2018, 12 (2), pp.e854-e866. ⟨10.1002/term.2396⟩
ISSN: 1932-7005
1932-6254
DOI: 10.1002/term.2396
Popis: International audience; Calcium phosphate (CaP)-based biomaterials are commonly used in bone reconstructive surgery to replace the damaged tissue, and can also serve as vectors for local drug delivery. Due to its inhibitory action on osteoclasts, the semi-metallic element gallium (Ga) is used for the systemic treatment of disorders associated with accelerated bone resorption. As it was demonstrated that Ga could be incorporated in the structure of CaP biomaterials, we investigated the biological properties of Ga-loaded CaP biomaterials. Culturing bone cells on Ga-CaP, we observed a decrease in osteoclast number and a downregulation of late osteoclastic markers expression, while Ga-CaP upregulated the expression of osteoblastic marker genes involved in the maturation of bone matrix. We next investigated in vivo bone reconstructive properties of different Ga-loaded biomaterials using a murine bone defect healing model. All implanted biomaterials showed a good osseointegration into the surrounding host tissue, accompanied by a successful bone ingrowth and bone marrow reconstruction, as evidenced by histological analysis. Moreover, quantitative micro-computed tomography analysis of implants revealed that Ga enhanced total defect filling. Lastly, we took advantage for the first time of a particular mode of non-linear microscopy (second harmonic generation) to quantify in vivo bone tissue reconstruction within a CaP bone substitute. By doing so, we showed that Ga exerted a positive impact on mature organized collagen synthesis. As a whole, our data support the hypothesis that Ga represents an attractive additive to CaP biomaterials for bone reconstructive surgery. Copyright © 2017 John Wiley & Sons, Ltd.
Databáze: OpenAIRE
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