Non-invasive grading of astrocytic tumours from the relative contents of myo-inositol and glycine measured by in vivo mrs
| Autor: | Ana Paula Candiota, Majós C, Julià-Sapé M, Cabañas M, Jj, Acebes, Moreno-Torres A, Jr, Griffiths, Arús C |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
lcsh:Medical physics. Medical radiology. Nuclear medicine
Pathology medicine.medical_specialty Magnetic Resonance Spectroscopy Biopsy lcsh:R895-920 Glycine Contrast Media Astrocytoma Statistics Nonparametric Choline chemistry.chemical_compound Nuclear magnetic resonance In vivo medicine Humans Inositol Astrocytoma - Magnetic resonance (MR) spectroscopy Astrocytoma - Magnetic resonance (MR) Grading (tumors) Spectroscopy Analysis of Variance Perchlorates Brain Neoplasms Phantoms Imaging business.industry Non invasive Creatine medicine.disease In vitro chemistry Neoplasm Grading business Anaplastic astrocytoma Glioblastoma |
| Zdroj: | Journal of the Belgian Society of Radiology; Vol 94, No 6 (2011); 319-329 Scopus-Elsevier Europe PubMed Central Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Journal of the Belgian Society of Radiology, Vol 94, Iss 6, Pp 319-329 (2011) |
| ISSN: | 1780-2393 |
| Popis: | Altres ajuts: INTERPRET (EU-IST1999-10310). This work was also partially funded by the Centro de Investigación Biomédica en Red - Bioingeniería, Biomateriales y Nanomedicina, which is an initiative of the Instituto de Salud Carlos III (Spain) co-funded by EU FEDER funds. MRI and MRS are established methodologies for evaluating intracranial lesions. One MR spectral feature suggested for in vivo grading of astrocytic tumours is the apparent myo-Inositol (mI) intensity (ca 3.55ppm) at short echo times, although glycine (gly) may also contribute in vivo to this resonance. The purpose of this study was to quantitatively evaluate the mI + gly contribution to the recorded spectral pattern in vivo and correlate it with in vitro data obtained from perchloric acid extraction of tumour biopsies. Patient spectra (n = 95) at 1.5T at short (20-31 ms) and long (135-136 ms) echo times were obtained from the INTERPRET MRS database (http://gabrmn.uab.es/interpretvalidateddb/). Phantom spectra were acquired with a comparable protocol. Spectra were automatically processed and the ratios of the (mI + gly) to Cr peak heights ((mI + gly)/Cr) calculated. Perchloric acid extracts of brain tumour biopsies were analysed by high-resolution NMR at 9.4T. The ratio (mI + gly)/Cr decreased significantly with astrocytic grade in vivo between low-grade astrocytoma (A2) and glioblastoma multiforme (GBM). In vitro results displayed a somewhat different tendency, with anaplastic astrocytomas having significantly higher (mI + gly)/Cr than A2 and GBM. The discrepancy between in vivo and in vitro data suggests that the NMR visibility of glycine in glial brain tumours is restricted in vivo. |
| Databáze: | OpenAIRE |
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