Laser Raman Spectroscopic Analysis of Polymorphic Forms in Microliter Fluid Volumes
| Autor: | Colin J. H. Brenan, Claudia Marcolli, Ian W. Hunter, Patrick A. Anquetil |
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| Rok vydání: | 2003 |
| Předmět: |
In situ
Laser raman Chemistry Lasers Microfluidics Analytical chemistry Pharmaceutical Science Recrystallization (metallurgy) Spectrum Analysis Raman Mass spectrometry Pharmaceutical Solutions symbols.namesake chemistry.chemical_compound symbols Technology Pharmaceutical Methanol Crystallization Raman spectroscopy |
| Zdroj: | Journal of Pharmaceutical Sciences. 92:149-160 |
| ISSN: | 0022-3549 |
| DOI: | 10.1002/jps.10267 |
| Popis: | Knowledge and control of the polymorphic phase of chemical compounds are important aspects of drug development in the pharmaceutical industry. We report herein in situ and real-time Raman spectroscopic polymorphic analysis of optically trapped microcrystals in a microliter volume format. The system studied in particular was the recrystallization of carbamazepine (CBZ) in methanol. Raman spectrometry enabled noninvasive measurement of the amount of dissolved CBZ in a sample as well as polymorphic characterization, whereas exclusive recrystallization of either CBZ form I or CBZ form III from saturated solutions was achieved by specific selection of sample cell cooling profiles. Additionally, using a microcell versus a macroscopic volume gives the advantage of reaching equilibrium much faster while using little compound quantity. We demonstrate that laser Raman spectral polymorphic analysis in a microliter cell is a potentially viable screening platform for polymorphic analysis and could lead to a new high throughput method for polymorph screening. |
| Databáze: | OpenAIRE |
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