Chronic Feline Leukemia Virus Infection Alters Arachidonic Acid Proportions In Vivo and In Vitro
| Autor: | Louis J. Lafrado, Mark G. Lewis, Lloyd A. Horrocks, Lowell L. Williams, Jennifer L. Rojko, Richard G. Olsen |
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| Rok vydání: | 1993 |
| Předmět: |
Chromatography
Gas Time Factors Lymphoma viruses animal diseases Cell Feline leukemia virus General Biochemistry Genetics and Molecular Biology Virus Microbiology chemistry.chemical_compound Immune system hemic and lymphatic diseases Immunopathology Tumor Cells Cultured medicine Animals chemistry.chemical_classification Arachidonic Acid Leukemia Experimental biology Leukemia Virus Feline Fatty Acids Fatty acid Thymus Neoplasms Lipid Metabolism biology.organism_classification Lipids medicine.anatomical_structure chemistry Viral replication Immunology Cats Arachidonic acid |
| Zdroj: | Experimental Biology and Medicine. 202:239-245 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-202-43533 |
| Popis: | The polyunsaturated omega-6 fatty acid, arachidonic acid ([AA] 20:4n-6), is both the key of the immunoregulatory substances, prostaglandins, and leukotrienes, and an essential component of immune cell membrane phospholipids, providing stability and flexibility to ensure cellular function. To explore possible effects of the physiological burden of viral replication in chronic viral infections on AA availability, plasma total esterified fatty acid (FA) proportions were measured in the feline leukemia (FeLV) model. Plasma FA profiles of 12 specific-pathogen-free cats with chronic infections with Rickard strain feline leukemia virus (FeLV-R) were compared with 12 age- and sex-matched uninfected specific-pathogen-free cats at 4 months after infection. A significant decrease from normal of average AA proportion was found in FeLV-R-infected cat plasma, while other major FA (palmitic, stearic, and oleic and omega-3 FA normally remained present until near death. Since plasma FA content rapidly affects circulating immune cell membrane composition and since FeLV infection also targets immune cells, we compared FA profiles of feline T4-thymic lymphoma 3201 cell membranes that were infected with virulent FeLV-R or less virulent FeLV-A, at 20 days after viral inoculation with sham-inoculated uninfected 3201 cells. Significantly altered FA proportions and ratios of saturated to unsaturated FA found in infected cell membranes were similar to plasma FA changes and paralleled the virulence of the FeLV inoculum. Altered postinfection FA proportions may impart serious functional defects to the immune cells of chronic FeLV-infected cats, contributing to the inability of their immune systems to eliminate FeLV by depleted plasma AA stores and modified cell membrane composition. Decreased AA availability may be an important factor in the cachexia and fatal outcome of FeLV infection. |
| Databáze: | OpenAIRE |
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