Characterization of whole genome radiation hybrid mapping resources for non-mammalian vertebrates
| Autor: | Maria E. Davis, Barry H. Paw, Ricky Critcher, Richard M. Korn, Linda McCarthy, Cheni Kwok, Peter N. Goodfellow, Dave Burt, Karin Schmitt, Leonard I. Zon |
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| Rok vydání: | 1998 |
| Předmět: |
Animal Genetics
ved/biology.organism_classification_rank.species Computational biology Biology Hybrid Cells Genome Polymerase Chain Reaction Cell Line chemistry.chemical_compound Gene mapping Cricetinae Genetics Animals Radiation hybrid mapping Model organism Zebrafish In Situ Hybridization Fluorescence Hybrid ved/biology Chromosome Mapping biology.organism_classification chemistry Chickens DNA Research Article |
| Zdroj: | Kwok, C, Korn, R M, Davis, M E, Burt, D W, Critcher, R, McCarthy, L, Paw, B H, Zon, L I, Goodfellow, P N & Schmitt, K 1998, ' Characterization of whole genome radiation hybrid mapping resources for non-mammalian vertebrates ', Nucleic Acids Research, vol. 26, no. 15, pp. 3562-6 . https://doi.org/10.1093/nar/26.15.3562 |
| ISSN: | 0305-1048 |
| Popis: | Radiation hybrid panels are already available for genome mapping in human and mouse. In this study we have used two model organisms (chicken and zebrafish) to show that hybrid panels that contain a full complement of the donor genome can be generated by fusion to hamster cells. The quality of the resulting hybrids has been assessed using PCR and FISH. We confirmed the utility of our panels by establishing the percentage of donor DNA present in the hybrids. Our hybrid resources will allow inexpensive gene mapping and we expect that this technology can be transferred to many other species. Such successes are providing the basis for a new era of mapping tools, in the form of whole genome radiation hybrid panels, and are opening new possibilities for systematic genome analysis in the animal genetics community. |
| Databáze: | OpenAIRE |
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