Evaluating Colombian Clostridium spp. Strains hydrogen production using glycerol as substrate
| Autor: | Luz Torres, Lizeth K Tinoco, M Mauricio Bernal, Dolly Montoya, Dionisio Malagón-Romero |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
glycerol
hydrogen Hydrogen anaerobic fermentation Chemistry Bioconversion Batch reactor Substrate (chemistry) chemistry.chemical_element Clostridium spp Applied Microbiology and Biotechnology Solvent chemistry.chemical_compound Biochemistry Glycerol biofuel Fermentation Food science mathematical model Biotechnology Hydrogen production |
| Zdroj: | Electronic Journal of Biotechnology v.16 n.2 2013 SciELO Chile CONICYT Chile instacron:CONICYT Electronic Journal of Biotechnology, Volume: 16, Issue: 2, Pages: 5-5, Published: 15 MAR 2013 |
| Popis: | Background: The ability for hydrogen production of 13 native strains of Clostridium spp. strains isolated from Colombian soil was evaluated using glycerol substrate. Glycerol to hydrogen conversion was investigated using a batch fermentation reactor and industrial glycerol source (50 g.l -1 , pH 7.00). Results: The results were quantified regarding acids, hydrogen, biomass and solvent production. The selected strain gave good hydrogen over production output at 14.4 mmol H 2 .l -1 , productivity 0.3167 mg H 2 .h -1 l - 1 culture mediumand yield 0.1962 mol H 2 .mol -1 glycerol. A further fermentation assay a 4.0 liter batch reactor let to being 0.26 mg.l -1 .h -1 after 18 hrs of fermentation. Logistic model, Luedeking-Piret model and Luedeking-Piret modified models were used for modeling changes in cell growth, hydrogen production and substrate consumption (Correlation coefficients R 2 = 0.95 for biomass substrate, R 2 = 0.77 hydrogen production). Conclusions: Our results indicate that hydrogen production through glycerol bioconversion by native strains is possible. |
| Databáze: | OpenAIRE |
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