Intralipid 10%: physicochemical characterization
| Autor: | Nicole Domingo, Jean-Claude Dedieu, Fabien Milliat, Suzanne Dunel-Erb, Claudine Chevalier, André Bach, Annie Gulik, Jacqueline Férézou |
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| Rok vydání: | 2001 |
| Předmět: |
Fat Emulsions
Intravenous Liposome Nutrition and Dietetics Chromatography Density gradient Chemistry Endocrinology Diabetes and Metabolism Bilayer Blotting Western Phospholipid Proteins Mesophase Chemical Fractionation Infranatant Microscopy Electron chemistry.chemical_compound Liposomes Centrifugation Density Gradient Humans Centrifugation Particle size Particle Size Triglycerides |
| Zdroj: | Nutrition. 17:930-933 |
| ISSN: | 0899-9007 |
| Popis: | OBJECTIVES: Parenteral fat emulsions contain two populations of particles: artificial chylomicrons rich in triacylglycerols (TAG), and liposomes (bilayer of phospholipids [PL] enveloping an aqueous phase). Centrifugation permits isolating the liposomes in the infranatant called mesophase . The aim of the present work was to better characterize this mesophase chemically and to view the particles it contains by electron microscopy. METHODS: Electron microscopy (Philips 410) was performed after cryofracture on native 10% Intralipid, mesophase (centrifugation for 1 h at 27 000 g ), and a liposome-enriched fraction (ring of density 1.010–1.030 g/l obtained after centrifuging mesophase in a KBr density gradient at 100 000 g for 24 h). The TAG and protein content of the mesophase was analyzed and the proteins partially characterized by immunodetection (Western-blot). RESULTS: This electron microscope study of 10% Intralipid gives evidence for the coexistence of artificial chylomicrons (mean diameter, 260 nm) and liposomes (43 nm), the latter being smaller than expected and containing 8% w/w TAG after purification. The solubilization of TAG in PL bilayers (reported to be ≤3.1% w/w) might have been increased in parenteral emulsions by the manufacturing process or/and the high TAG/PL ratio. Minute amounts of proteins have also been detected and partially characterized using a specific antibody raised against the human 7 kDa Anionic Polypeptide Factor (APF), known to strongly interact with PL in bile. CONCLUSIONS: This work has shown that the size (mean diameter, 43 nm) of the liposomes present in 10% Intralipid is smaller than that usually assumed. Traces of hydrophobic proteins in the emulsion may account for certain allergic reactions sometimes observed in infused patients. |
| Databáze: | OpenAIRE |
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