Oxidized low-density lipoprotein increases the production of intracellular reactive oxygen species in endothelial cells
| Autor: | A. M. Pastorino, G. Gaviraghi, Anna Fratta Pasini, Anna Davoli, Luciano Cominacini, V. Lo Cascio, M. Campagnola, Ulisse Garbin |
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| Rok vydání: | 1998 |
| Předmět: |
Intracellular Fluid
Dihydropyridines Endothelium Arteriosclerosis Physiology Antioxidants chemistry.chemical_compound Dichlorofluorescein Internal Medicine medicine Humans Receptor Cells Cultured Fluorescent Dyes Cell adhesion molecule business.industry NF-kappa B Calcium Channel Blockers Fluoresceins Cell biology Lipoproteins LDL Endothelial stem cell medicine.anatomical_structure Biochemistry Lacidipine chemistry Endothelium Vascular Signal transduction Reactive Oxygen Species Cardiology and Cardiovascular Medicine business Cell Adhesion Molecules Oxidation-Reduction Intracellular medicine.drug |
| Zdroj: | Journal of Hypertension. 16:1913-1919 |
| ISSN: | 0263-6352 |
| DOI: | 10.1097/00004872-199816121-00010 |
| Popis: | Objective The mechanisms by which oxidized low-density lipoprotein (ox-LDL) induces the expression of adhesion molecules on endothelial cells (HUVECs) are still not clear. The signal transduction pathways for these binding molecules include the translocation of the transcription factor NF-kB and the intracellular reactive oxygen species (ROS) are said to play a key role in this process. Aim of this study was (1) to evaluate the effect of ox-LDL on intracellular production of ROS in culture of HUVECs; (2) to evaluate if the intracellular increase of ROS induced by ox-LDL is mediated by the binding to a specific endothelial receptor; (3) to ascertain if lacidipine can decrease ox-LDL-induced ROS production in HUVECs. Methods Five μM Cu 2+ ox-LDL were incubated with HUVECs for 5 min. 2',7'-Dichlorofluorescein (DCF) as an expression of intracellular ROS production, was measured by flow cytometry. Results ox-LDL induced a significant dose-dependent increase in DCF production (P< 0.001) through the binding to a specific receptor. The preincubation of HUVECs with radical scavengers compounds and lacidipine significantly reduced (P |
| Databáze: | OpenAIRE |
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