Characterization of a strong constitutive promoter from paper mulberry vein banding virus
| Autor: | Jing Yu, Peng Zhang, Yu Ma, Yong-Yuan Cheng, Quan-You Lu, William Kojo Smith, Tao-Tao Han |
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| Rok vydání: | 2021 |
| Předmět: |
Genetics
Expression vector biology Transgene fungi food and beverages Nicotiana benthamiana General Medicine Broussonetia biology.organism_classification Plants Genetically Modified Virology Genome Badnavirus Caulimovirus Tobacco Arabidopsis thaliana Cauliflower mosaic virus Morus Promoter Regions Genetic |
| Zdroj: | Archives of virology. 167(1) |
| ISSN: | 1432-8798 |
| Popis: | Paper mulberry vein banding virus (PMVBV), a member of the genus Badnavirus in the family Caulimoviridae, infects paper mulberry (Broussonetia papyrifera), a dicotyledonous plant. Putative promoter regions in the PMVBV genome were tested using recombinant plant expression vectors, revealing that the promoter activity of three genome fragments was about 1.5-fold higher than that of the 35S promoter of cauliflower mosaic virus in Nicotiana benthamiana. In transformed transgenic Arabidopsis thaliana plants, these promoter constructs showed constitutive expression. Based on the activity and gene expression patterns of these three promoter constructs, a fragment of 384 bp (named PmVP) was deduced to contain the full-length promoter of the PMVBV genome. The results suggest that the PMVBV-derived promoter can be used for the constitutive expression of transgenes in dicotyledonous plants. |
| Databáze: | OpenAIRE |
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