Effect of delta opioid receptor activation on spatial cognition and neurogenesis in cerebral ischemic rats
| Autor: | Bing Zhao, Yale Duan, Guangming Zhang, Shuyan Wang, Xiang-Rui Wang, Zheng Zhao |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Agonist medicine.drug_class Neurogenesis Spatial Learning Ischemia Morris water navigation task Apoptosis Pharmacology Brain Ischemia Rats Sprague-Dawley δ-opioid receptor Brain ischemia 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Naltrindole Receptors Opioid delta medicine Animals Maze Learning Cell Proliferation Neurons business.industry General Neuroscience Brain Cell Differentiation Enkephalin Leucine-2-Alanine medicine.disease Neuroprotective Agents 030104 developmental biology chemistry DADLE business Neuroscience 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Neuroscience Letters. 620:20-26 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/j.neulet.2016.03.035 |
| Popis: | This study aimed to investigate whether a selective delta opioid receptor agonist, [D-Ala2, D-Leu5]-Enkephalin (DADLE), regulates neurogenesis in the hippocampus of ischemic rats. Using an intracerebral cannula, rats were subjected to cerebral ischemia using the standard four-vessel occlusion. DADLE (2.5nmol), DADLE (2.5nmol) with naltrindole (NAL) (2.5nmol), or vehicle was administered at the onset of reperfusion. Bromodeoxyuridine (BrdU, 100mg/kg, intraperitoneal) was used to label newly formed cells from days 1 to 7 after ischemia. Immunohistochemistry was used to evaluate cell proliferation and apoptosis and differentiation 7days 28 days, respectively, after ischemia. Morris water maze test was conducted to test spatial learning and memory 23-27 days after ischemia. We found that DADLE treatment improved performance in the Morris water maze test, promoted proliferation and differentiation of newly formed neurons, and inhibited differentiation into astrocytes in a rat model of cerebral ischemia. Furthermore, the protective effects of DADLE were significantly reversed by co-administration of NAL (P |
| Databáze: | OpenAIRE |
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