Auxotrophic Mutations Reduce Tolerance of Saccharomyces cerevisiae to Very High Levels of Ethanol Stress
| Autor: | Pieter Verdyck, Steve Swinnen, Johan M. Thevelein, Kristien Schaerlaekens, Annelies Goovaerts, Kris Souvereyns, Françoise Dumortier, Maria R. Foulquié-Moreno, Griet Van Zeebroeck |
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| Přispěvatelé: | Medical Genetics, Reproduction and Genetics |
| Rok vydání: | 2015 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Auxotrophy Saccharomyces cerevisiae Microbiology chemistry.chemical_compound Nutrient Drug Resistance Fungal Stress Physiological Ethanol/toxicity Amino Acids/genetics Amino Acids Drug Resistance Fungal/genetics Molecular Biology Ethanol biology Fungal genetics Uracil Articles General Medicine biology.organism_classification Yeast chemistry Biochemistry Mutation Saccharomyces cerevisiae Proteins/genetics Leucine Saccharomyces cerevisiae/drug effects |
| Zdroj: | Eukaryotic Cell. 14:884-897 |
| ISSN: | 1535-9786 1535-9778 |
| DOI: | 10.1128/ec.00053-15 |
| Popis: | Very high ethanol tolerance is a distinctive trait of the yeast Saccharomyces cerevisiae with notable ecological and industrial importance. Although many genes have been shown to be required for moderate ethanol tolerance (i.e., 6 to 12%) in laboratory strains, little is known of the much higher ethanol tolerance (i.e., 16 to 20%) in natural and industrial strains. We have analyzed the genetic basis of very high ethanol tolerance in a Brazilian bioethanol production strain by genetic mapping with laboratory strains containing artificially inserted oligonucleotide markers. The first locus contained the ura3 Δ 0 mutation of the laboratory strain as the causative mutation. Analysis of other auxotrophies also revealed significant linkage for LYS2 , LEU2 , HIS3 , and MET15 . Tolerance to only very high ethanol concentrations was reduced by auxotrophies, while the effect was reversed at lower concentrations. Evaluation of other stress conditions showed that the link with auxotrophy is dependent on the type of stress and the type of auxotrophy. When the concentration of the auxotrophic nutrient is close to that limiting growth, more stress factors can inhibit growth of an auxotrophic strain. We show that very high ethanol concentrations inhibit the uptake of leucine more than that of uracil, but the 500-fold-lower uracil uptake activity may explain the strong linkage between uracil auxotrophy and ethanol sensitivity compared to leucine auxotrophy. Since very high concentrations of ethanol inhibit the uptake of auxotrophic nutrients, the active uptake of scarce nutrients may be a major limiting factor for growth under conditions of ethanol stress. |
| Databáze: | OpenAIRE |
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