Post-translational regulation of Saccharomyces cerevisiae proteins tagged with the hormone-binding domains of mammalian nuclear receptors
| Autor: | H. Launhardt, T. Munder |
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| Rok vydání: | 2000 |
| Předmět: |
Time Factors
Saccharomyces cerevisiae Ligands Models Biological Open Reading Frames Receptors Glucocorticoid Transformation Genetic Genetics Animals Post-translational regulation ORFS Receptor Molecular Biology Cell Nucleus Models Genetic biology General Medicine biology.organism_classification Fusion protein Hormones Yeast Protein Structure Tertiary Cell biology Kinetics Receptors Estrogen Nuclear receptor Mutagenesis Protein Processing Post-Translational Cell Division Function (biology) Plasmids |
| Zdroj: | Molecular Genetics and Genomics. 264:317-324 |
| ISSN: | 1617-4623 1617-4615 |
| DOI: | 10.1007/s004380000325 |
| Popis: | In the post-genome sequencing era the functional analysis of newly discovered proteins becomes more and more important. In this report we describe a genetic approach to the post-translational regulation of protein function in Saccharomyces cerevisiae by creating conditional lethal mutants. The yeast ORFs YDL139c, YDL147w, ERG3 and ERG11 were tagged with sequences encoding the hormone-binding domains of mammalian steroid receptors by PCR-mediated, targeted integration into the yeast genome. We found that the function of the chimeric proteins is regulated in a hormone-dependent way. This technique provides another important tool for the functional analysis of the yeast proteome. |
| Databáze: | OpenAIRE |
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