Intrauterine embryo transfer with canine embryos cryopreserved by the slow freezing and the Cryotop method
| Autor: | Taku Kimura, Tatsuya Hori, Masanori Kobayashi, Eiichi Kawakami, Toshihiko Tsutsui, Hitoshi Ushijima |
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| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
animal structures Cryoprotectant Uterus embryo Biology Cryopreservation Time Andrology 03 medical and health sciences slow freezing method 0302 clinical medicine Cryoprotective Agents Dogs Embryo cryopreservation Pregnancy medicine Animals Blastocyst Gynecology Cryotop method 030219 obstetrics & reproductive medicine General Veterinary Full Paper 0402 animal and dairy science Embryo Uterine horns 04 agricultural and veterinary sciences Embryo Transfer 040201 dairy & animal science Embryo transfer medicine.anatomical_structure embryonic structures dog Female Theriogenology |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 |
| Popis: | Canine embryos (8-cell to blastocyst stages) frozen-thawed using the slow-freezing method with glycerol (four recipients) or dimethyl sulfoxide (three recipients) as a cryoprotectant and vitrified-warmed using the Cryotop method (five recipients) were surgically transferred into the unilateral uterine horn of recipient bitches. As a result, the morphology of embryos frozen-thawed using the slow-freezing method was judged to be normal, but no conception occurred in any of the recipient bitches. Two of the five bitches that received transferred embryos (morula to early blastocyst stages) vitrified-warmed using the Cryotop method became pregnant and produced normal pups (1/9 embryos, 11.1% and 1/6 embryos, 17.0%). It was concluded that the Cryotop method was more appropriate for canine embryo cryopreservation than the slow-freezing method, which is used for the cryopreservation of embryos of other mammalian species. |
| Databáze: | OpenAIRE |
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