Physiological and biochemical responses of soybean plants inoculated with Arbuscular mycorrhizal fungi and Bradyrhizobium under drought stress
| Autor: | Ahmed M. El-Sawah, Milan Skalicky, Dina Fathi Ismail Ali, Hiba Shaghaleh, Yousef Alhaj Hamoud, Muhammad Farooq, You-Cai Xiong, Anket Sharma, Zaid Ulhassan, Mohamed S. Sheteiwy, Marian Brestic, Hamada AbdElgawad |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Biofertilizer Soil enzymes Plant Science 01 natural sciences Bradyrhizobium 03 medical and health sciences Stress Physiological Mycorrhizae AMF parasitic diseases Proline Flow cytometry Soybean yield Secondary metabolism Fertilizers Rhizosphere biology Research fungi Botany Sowing food and beverages biology.organism_classification Droughts Horticulture 030104 developmental biology QK1-989 Soybeans Proline metabolism Soil fertility 010606 plant biology & botany Bradyrhizobium japonicum |
| Zdroj: | BMC Plant Biology, Vol 21, Iss 1, Pp 1-21 (2021) BMC Plant Biology |
| ISSN: | 1471-2229 |
| Popis: | Background The present study aims to study the effects of biofertilizers potential of Arbuscular Mycorrhizal Fungi (AMF) and Bradyrhizobium japonicum (B. japonicum) strains on yield and growth of drought stressed soybean (Giza 111) plants at early pod stage (50 days from sowing, R3) and seed development stage (90 days from sowing, R5). Results Highest plant biomass, leaf chlorophyll content, nodulation, and grain yield were observed in the unstressed plants as compared with water stressed-plants at R3 and R5 stages. At soil rhizosphere level, AMF and B. japonicum treatments improved bacterial counts and the activities of the enzymes (dehydrogenase and phosphatase) under well-watered and drought stress conditions. Irrespective of the drought effects, AMF and B. japonicum treatments improved the growth and yield of soybean under both drought (restrained irrigation) and adequately-watered conditions as compared with untreated plants. The current study revealed that AMF and B. japonicum improved catalase (CAT) and peroxidase (POD) in the seeds, and a reverse trend was observed in case of malonaldehyde (MDA) and proline under drought stress. The relative expression of the CAT and POD genes was up-regulated by the application of biofertilizers treatments under drought stress condition. Interestingly a reverse trend was observed in the case of the relative expression of the genes involved in the proline metabolism such as P5CS, P5CR, PDH, and P5CDH under the same conditions. The present study suggests that biofertilizers diminished the inhibitory effect of drought stress on cell development and resulted in a shorter time for DNA accumulation and the cycle of cell division. There were notable changes in the activities of enzymes involved in the secondary metabolism and expression levels of GmSPS1, GmSuSy, and GmC-INV in the plants treated with biofertilizers and exposed to the drought stress at both R3 and R5 stages. These changes in the activities of secondary metabolism and their transcriptional levels caused by biofertilizers may contribute to increasing soybean tolerance to drought stress. Conclusions The results of this study suggest that application of biofertilizers to soybean plants is a promising approach to alleviate drought stress effects on growth performance of soybean plants. The integrated application of biofertilizers may help to obtain improved resilience of the agro ecosystems to adverse impacts of climate change and help to improve soil fertility and plant growth under drought stress. |
| Databáze: | OpenAIRE |
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