The influence of epithelia on cartilage and loose connective tissue formation by limb mesenchyme cultures
| Autor: | Michael Solursh, Carl T. Singley, Rebecca S. Reiter |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Apical ectodermal ridge
animal structures Mesenchyme Connective tissue Ectoderm Chick Embryo Biology Epithelium Somatopleuric mesenchyme Limb bud medicine Animals Wings Animal Molecular Biology Cells Cultured Connective Tissue Cells Cell Differentiation Cell Biology Anatomy Chondrogenesis Cell biology medicine.anatomical_structure Cartilage Zone of polarizing activity Connective Tissue embryonic structures Collagen Developmental Biology |
| Zdroj: | Developmental biology. 86(2) |
| ISSN: | 0012-1606 |
| Popis: | In order to explain the observation that normally nonchondrogenic limb mesenchyme forms extensive cartilage in culture, the possibility that limb ectoderm inhibits chondrogenesis is examined. Small pieces of quail or chick ectoderm are grafted onto micromass cultures of wing mesenchyme from stage 23–34 chick embryos. The presence of nonridge wing or several other epithelia results in increased collagen accumulation in the underlying mesenchyme, a delay in cell differentiation, and the eventual formation of loose connective tissue, as determined by transmission electron microscopy. The influence can occur across Nuclepore filters having 0.1-μm-diameter pores and ultrathin Millipore filters, but not across Millipore filters of standard thickness. The influence is not contact dependent since cell processes do not cross these filters. The apical ectodermal ridge has the additional effect of stimulating mesenchymal outgrowth. These results support the idea that the ectoderm plays a direct but negative role in the formation of a chondrogenic core within the developing limb. |
| Databáze: | OpenAIRE |
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