Molecular cloning of two west African HIV2 isolates that replicate well in macrophages
| Autor: | Ursula Dietrich, H. von Briesen, C. Meichsner, Renate Kreutz, A Seipp, Herbert Kühnel, H. Rübsamen-Waigmann, Michalina Adamski |
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| Rok vydání: | 1990 |
| Předmět: |
Syncytium
Base Sequence Strain (chemistry) Macrophages Molecular Sequence Data Immunology DNA Recombinant Nucleic acid sequence RNA-Directed DNA Polymerase Biology Molecular cloning Virus Replication Virology Molecular biology Peripheral blood Reverse transcriptase Africa Western West african Sequence Homology Nucleic Acid DNA Viral HIV-2 Humans Cloning Molecular |
| Zdroj: | Research in Virology. 141:233-237 |
| ISSN: | 0923-2516 |
| DOI: | 10.1016/0923-2516(90)90026-f |
| Popis: | HIV2 strains were isolated from a Gambian with neuro-AIDS (HIV2 D194 ) and from an asymptomatic Ghanian (HIV2 D205 ). Like HIV1 biological subtype c, both isolates grew slowly and induced few or no syncytia, but eventually produced high levels of particle-associated reverse transcriptase (RT) in cultures of fresh peripheral blood lymphocytes. Each produced even higher levels of RT in fresh human macrophages, especially HIV2 D194 , where maximal RT values of 1,800,000 cpm/ml supernatant of approximately 30,000 cells were measured. The viruses were molecularly cloned after a single passage in culture. Restriction-site analysis showed heterogeneity within each isolate. Nucleotide sequence analysis of HIV2 D194 revealed that, genetically, it is a member of the prototypic HIV2 family, displaying 12% divergence vs. HIV2 ROD and HIV2 NIHZ . In contrast, HIV2 D205 is the most highly divergent HIV2 strain yet described: it is equidistant in relation between the known HIV2 strains and the SIV MAC isolates (23–24 % nucleotide sequence divergence). |
| Databáze: | OpenAIRE |
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