Prostaglandin E2 promotes human CD34+ cells homing through EP2 and EP4 in vitro

Autor: Shuping Lai, Duo-Rong Xu, Hui-Zhen Chen, Chang Su, Jing Tang, Ya-Qun Wang, Waiyi Zou, Chun Feng, Fang-Jie Liu
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Cancer Research
Receptors
CXCR4
Stromal cell
Prostaglandin E2 receptor
Gene Expression
Antigens
CD34
Biology
Biochemistry
Dinoprostone
Immunophenotyping
03 medical and health sciences
Chemokine receptor
Cell Movement
Genetics
Humans
allogeneic hematopoietic stem cell transplantation
Receptor
Molecular Biology
Cells
Cultured
PGE2 receptor
Cluster of differentiation
PGE2 receptor agonist
Mesenchymal stem cell
implantation dysfunction
Hematopoietic Stem Cell Transplantation
Cell migration
Mesenchymal Stem Cells
Articles
Receptors
Prostaglandin E
EP2 Subtype
Hematopoietic Stem Cells
Chemokine CXCL12
Cell biology
030104 developmental biology
Phenotype
Oncology
Gene Expression Regulation
Molecular Medicine
lipids (amino acids
peptides
and proteins)
homing
Receptors
Prostaglandin E
EP4 Subtype
Biomarkers
Homing (hematopoietic)
Zdroj: Molecular Medicine Reports
ISSN: 1791-3004
1791-2997
Popis: Recently, certain studies have demonstrated in vitro that prostaglandin E2 (PGE2) promotes human cluster of differentiation (CD)34+ cell homing. However, the sub‑type receptors activated by PGE2 are unknown, as the PGE2 receptor EP1-4 subtypes (EP1-4) are expressed on the membrane of human CD34+ cells. Based on the above, the present study aimed to screen the receptor subtype activity by PGE2 to promote human CD34+ cell homing. It was observed that human CD34+ cells expressed the four PGE2 sub‑receptors, particularly EP2 and 4. PGE2 increased EP2 and 4 mRNA expression significantly, while EP1 and 3 mRNA exhibited no significant alteration. PGE2, EP2 agonist (EP2A), and EP4A upregulated C‑X‑C chemokine receptor 4 mRNA and protein expression in human CD34+ cells, and promoted stromal cell‑derived factor 1α (SDF‑1α) expression in bone marrow mesenchymal stem cells (BMMSCs). These phenomena were inhibited by the associated receptor antagonists. PGE2, EP2A, and EP4A facilitated human CD34+ cell migration towards SDF‑1α and BMMSCs. The results of the present study suggested that PGE2 promoted human CD34+ cell homing through EP2 and 4 receptors in vitro.
Databáze: OpenAIRE
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