Aesculetin Accelerates Osteoblast Differentiation and Matrix-Vesicle-Mediated Mineralization

Autor: Moon-Sik Oh, Dong Yeon Kim, Woojin Na, Sin-Hye Park, Min-Kyung Kang, Young-Hee Kang, Sohyun Park, Ii-Jun Kang, Su Yeon Oh
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Bone sialoprotein
Bone Matrix
Core Binding Factor Alpha 1 Subunit
Matrix (biology)
chemistry.chemical_compound
Mice
Osteogenesis
Osteonectin
Osteopontin
Biology (General)
Spectroscopy
biology
Chemistry
hydroxyapatite
Osteoblast
non-collagenous proteins
Cell Differentiation
General Medicine
Computer Science Applications
Cell biology
aesculetin
medicine.anatomical_structure
osteoblast differentiation
Aesculetin
Signal Transduction
QH301-705.5
Osteocalcin
Catalysis
Bone resorption
Article
matrix vesicles
Collagen Type I
Cell Line
Inorganic Chemistry
Extracellular Vesicles
Calcification
Physiologic
collagen mineralization
medicine
Animals
Integrin-Binding Sialoprotein
Umbelliferones
Physical and Theoretical Chemistry
Bone regeneration
Molecular Biology
QD1-999
Osteoblasts
Organic Chemistry
Osteoprotegerin
biology.protein
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 22, Iss 12391, p 12391 (2021)
Volume 22
Issue 22
ISSN: 1422-0067
Popis: The imbalance between bone resorption and bone formation in favor of resorption results in bone loss and deterioration of bone architecture. Osteoblast differentiation is a sequential event accompanying biogenesis of matrix vesicles and mineralization of collagen matrix with hydroxyapatite crystals. Considerable efforts have been made in developing naturally-occurring plant compounds, preventing bone pathologies, or enhancing bone regeneration. Coumarin aesculetin inhibits osteoporosis through hampering the ruffled border formation of mature osteoclasts. However, little is known regarding the effects of aesculetin on the impairment of matrix vesicle biogenesis. MC3T3-E1 cells were cultured in differentiation media with 1–10 μM aesculetin for up to 21 days. Aesculetin boosted the bone morphogenetic protein-2 expression, and alkaline phosphatase activation of differentiating MC3T3-E1 cells. The presence of aesculetin strengthened the expression of collagen type 1 and osteoprotegerin and transcription of Runt-related transcription factor 2 in differentiating osteoblasts for 9 days. When ≥1–5 μM aesculetin was added to differentiating cells for 15–18 days, the induction of non-collagenous proteins of bone sialoprotein II, osteopontin, osteocalcin, and osteonectin was markedly enhanced, facilitating the formation of hydroxyapatite crystals and mineralized collagen matrix. The induction of annexin V and PHOSPHO 1 was further augmented in ≥5 μM aesculetin-treated differentiating osteoblasts for 21 days. In addition, the levels of tissue-nonspecific alkaline phosphatase and collagen type 1 were further enhanced within the extracellular space and on matrix vesicles of mature osteoblasts treated with aesculetin, indicating matrix vesicle-mediated bone mineralization. Finally, aesculetin markedly accelerated the production of thrombospondin-1 and tenascin C in mature osteoblasts, leading to their adhesion to preformed collagen matrix. Therefore, aesculetin enhanced osteoblast differentiation, and matrix vesicle biogenesis and mineralization. These findings suggest that aesculetin may be a potential osteo-inductive agent preventing bone pathologies or enhancing bone regeneration.
Databáze: OpenAIRE
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