A simple interferon assay as an adjunct for determining the genus of origin of cell cultures
| Autor: | Aaron E. Freeman, Paul J. Price, Zenobia Holbrook, Carol P. Uhlendorf, Eugene M. Zimmerman |
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| Rok vydání: | 1972 |
| Předmět: |
Sindbis virus
Cell type Fibrosarcoma Cytological Techniques Newcastle disease virus Mice Inbred Strains Plant Science Kidney Vesicular stomatitis Indiana virus Cell Line Mice Cytopathogenic Effect Viral Species Specificity Interferon Rats Inbred BN biology.animal medicine Animals Humans Primate Skin biology Muscles Haplorhini Fibroblasts Orthomyxoviridae biology.organism_classification Virology In vitro Rats Vesicular stomatitis virus Cell culture Karyotyping Interferons Rabbits Sindbis Virus African Green Monkey Biotechnology medicine.drug |
| Zdroj: | In Vitro. 8:85-90 |
| ISSN: | 1475-2689 0073-5655 |
| Popis: | A short, simple test involving interferon-mediated protection of cells in vitro from cytopathogenic effects produced by vesicular stomatitis virus or Sindbis virus has been developed to help in determining the genus of origin of cells. By using the observed pattern of protection of five cell types by five interferons, cells could be grouped as of primate, rabbit, rat, and mouse origins. The primate grouping resulted from bilateral cross-reactions between the human and monkey systems. An unexpected observation was that African green monkey interferon preparations protect both monkey and rat cells, but not the converse. Chromosomal analysis of the cell cultures confirmed the genus determined by the interferon test. |
| Databáze: | OpenAIRE |
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