Functional role of miR-27b in the development of gastric cancer

Autor: Zhaohu Yuan, Ye-jia Cui, Xuhong Xie, Yaming Wei, Yanfen Xing, Xiaojie Chen
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
Cancer Research
Vascular Endothelial Growth Factor C
Apoptosis
Biochemistry
0302 clinical medicine
Neoplasm Metastasis
Articles
Middle Aged
Cell cycle
Gene Expression Regulation
Neoplastic
Cell Transformation
Neoplastic
Oncology
Vascular endothelial growth factor C
030220 oncology & carcinogenesis
Molecular Medicine
Female
Adult
proliferation
Biology
03 medical and health sciences
Stomach Neoplasms
Cell Line
Tumor
microRNA
Biomarkers
Tumor
Genetics
medicine
Humans
Circulating MicroRNA
receiver operating characteristic curve
Molecular Biology
Aged
Cell Proliferation
Neoplasm Staging
Oncogene
Cell growth
Gene Expression Profiling
gastric cancer
Cancer
medicine.disease
Molecular medicine
Gene expression profiling
MicroRNAs
microRNA-27b
030104 developmental biology
ROC Curve
Case-Control Studies
Cancer research
Neoplasm Grading
Zdroj: Molecular Medicine Reports
ISSN: 1791-3004
1791-2997
DOI: 10.3892/mmr.2018.8538
Popis: Previous studies have demonstrated that microRNAs (miRNAs/miRs) act as tumor suppressors or oncogenes during multiple processes in cancer. It has been observed that miR-27b may act as a tumor-suppressor and was significantly downregulated in a number of types of cancer. However, the functions of miR-27b in gastric cancer (GC) remain unclear. The present study aimed to investigate the functional role of miR-27b in the progression of GC. The downregulation of miR-27b in human GC plasma was confirmed using miRNA microarray and reverse transcription-quantitative polymerase chain reaction analyses. The association between circulating miR-27b expression and clinicopathological features of GC was analyzed and the results demonstrated that the level of circulating miR-27b was significantly correlated with GC differentiation. Receiver operating characteristic curve analysis identified that the plasma level of miR-27b may be a potential biomarker for differentiating patients with GC from healthy controls. In order to investigate the effect of miR-27b on GC cell behavior, miR-27b was overexpressed using miR-27b mimics, and it was observed that miR-27b was able to inhibit cell proliferation and induce apoptosis in SGC7901 cells. Previous studies have demonstrated that vascular endothelial growth factor C (VEGFC) is a target of miR-27b, and the results of the present study were consistent with these reports. Taken together, the results of the present study indicated that miR-27b may act as a potential biomarker for differentiating patients with GC from healthy controls, and serve as a tumor suppressor in GC by targeting VEGFC.
Databáze: OpenAIRE
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