RNA-template dependent de novo telomere addition
| Autor: | Patrick Philipp Weil, Franziska Jönsson, Jan Postberg, Hans J. Lipps, Gloria Fuhrmann |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
DNA Replication RNA Untranslated Nucleolus Biology Brief Communication Models Biological Germline 03 medical and health sciences chemistry.chemical_compound Ciliophora Molecular Biology RNA Double-Stranded Genetics 030102 biochemistry & molecular biology Macronucleus DNA replication Gene Amplification RNA Genetic Variation Cell Biology Templates Genetic Telomere Non-coding RNA 030104 developmental biology chemistry DNA Cell Nucleolus |
| Popis: | De novo addition of telomeric sequences can occur at broken chromosomes and must be well controlled, which is essential during programmed DNA reorganization processes. In ciliated protozoa an extreme form of DNA-reorganization is observed during macronuclear differentiation after sexual reproduction leading to the elimination of specific parts of the germline genome. Regulating these processes involves small noncoding RNAs, but in addition DNA-reordering, excision and amplification require RNA templates deriving from the parental macronucleus. We show that these putative RNA templates can carry telomeric repeats. Microinjection of RNA templates carrying modified telomeres into the developing macronucleus leads to modified telomeres in vegetative cells, providing strong evidence, that de novo addition of telomeres depends on a telomere-containing transcript from the parental macronucleus. |
| Databáze: | OpenAIRE |
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