Differentiating iron-loading anemias using a newly developed and analytically validated ELISA for human serum erythroferrone
| Autor: | Rachel P. L. van Swelm, Dorine W. Swinkels, Laura E. Diepeveen, Léon Kautz, Rian Roelofs, Nicolai Grebenchtchikov |
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| Přispěvatelé: | Radboud university [Nijmegen], Institut de Recherche en Santé Digestive (IRSD ), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), E.C. Noyons foundation, Radboud University [Nijmegen], SEGUIN, Nathalie |
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Ineffective erythropoiesis Erythroblasts Physiology Peptide Hormones medicine.disease_cause Matrix (chemical analysis) Mathematical and Statistical Techniques 0302 clinical medicine Sideroblastic anemia Animal Cells Red Blood Cells Medicine and Health Sciences Erythropoiesis Enzyme-Linked Immunoassays [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology Multidisciplinary biology Chemistry Statistics Drugs food and beverages Anemia Hematology Middle Aged Erythroferrone Body Fluids Women's cancers Radboud Institute for Health Sciences [Radboudumc 17] 3. Good health Blood Physical Sciences Regression Analysis Cytokines Medicine Female Anatomy Cellular Types Research Article Adult Analyte Iron Overload [SDV.SP.MED] Life Sciences [q-bio]/Pharmaceutical sciences/Medication [CHIM.ANAL] Chemical Sciences/Analytical chemistry Iron Science Bone Marrow Cells Enzyme-Linked Immunosorbent Assay Linear Regression Analysis Research and Analysis Methods Blood Plasma 03 medical and health sciences All institutes and research themes of the Radboud University Medical Center Hepcidins [SDV.SP.MED]Life Sciences [q-bio]/Pharmaceutical sciences/Medication [CHIM.ANAL]Chemical Sciences/Analytical chemistry Hepcidin medicine Humans Statistical Methods Immunoassays Aged Pharmacology Blood Cells Heparin beta-Thalassemia fungi Biology and Life Sciences Cell Biology medicine.disease Molecular biology Hematopoiesis Anemia Sideroblastic Renal disorders Radboud Institute for Molecular Life Sciences [Radboudumc 11] 030104 developmental biology Polyclonal antibodies Immunologic Techniques biology.protein Physiological Processes Mathematics [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology 030215 immunology |
| Zdroj: | PLoS One, 16 PLoS One, 16, 7 PLoS ONE PLoS ONE, Public Library of Science, 2021, 16 (7), 14 p. ⟨10.1371/journal.pone.0254851⟩ PLoS ONE, Vol 16, Iss 7, p e0254851 (2021) PLoS ONE, 2021, 16 (7), 14 p. ⟨10.1371/journal.pone.0254851⟩ |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0254851⟩ |
| Popis: | Erythroferrone (ERFE), the erythroid regulator of iron metabolism, inhibits hepcidin to increase iron availability for erythropoiesis. ERFE plays a pathological role during ineffective erythropoiesis as occurs in X-linked sideroblastic anemia (XLSA) and β-thalassemia. Its measurement might serve as an indicator of severity for these diseases. However, for reliable quantification of ERFE analytical characterization is indispensable to determine the assay’s limitations and define proper methodology. We developed a sandwich ELISA for human serum ERFE using polyclonal antibodies and report its extensive analytical validation. This new assay showed, for the first time, the differentiation of XLSA and β-thalassemia major patients from healthy controls (p = 0.03) and from each other (p2 = 0.83). Nevertheless, employment of one optimal dilution of all serum samples is warranted to obtain reliable results. When adequately performed, the assay can be used to further unravel the human erythropoiesis-hepcidin-iron axis in various disorders and assess the added diagnostic value of ERFE. |
| Databáze: | OpenAIRE |
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