Multilevel regulation of histone gene expression during the cell cycle in tobacco cells
| Autor: | Jean-Philippe Reichheld, Claude Gigot, Nicole Chaubet-Gigot |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
DNA Replication
Transcriptional Activation DNA Plant Transcription Genetic Molecular Sequence Data SAP30 Biology Histones Histone H1 Gene Expression Regulation Plant Histone H2A Histone methylation Tobacco Genetics Histone code RNA Processing Post-Transcriptional Promoter Regions Genetic Regulation of gene expression Histone deacetylase 5 Base Sequence Cell Cycle Molecular biology Plants Toxic Histone methyltransferase Protein Biosynthesis Research Article |
| Zdroj: | Nucleic acids research. 26(13) |
| ISSN: | 0305-1048 |
| Popis: | The respective involvement of transcriptional and post-transcriptional mechanisms in coupling H3 and H4 histone gene expression to the S phase of the cell cycle has been studied in synchronized tobacco cells. Induction of histone gene expression at the G1/S transition is shown to be essentially directed by an increase in the transcription rate in response to cellular signals occurring at the initiation step of DNA replication. Histone gene induction thus precedes the burst of DNA synthesis. However, when the elongation step of DNA replication is ineffective or artificially arrested, feedback mechanisms apparently act at the translation level to avoid overproduction of histone proteins from their mRNAs. At the end of S phase, post-transcriptional mechanisms ensure a rapid degradation of histone mRNAs. Transcription factors are bound to the cis -elements of histone promoters throughout the cell cycle, thus suggesting a post-translational modification of some of them to trigger promoter activation at the G1/S transition. Based on these results, a model is proposed for histone gene transcriptional induction in connection with the components of the cell cycle machinery. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|