Asymmetric Transcription of Bacteriophage Mu-1
| Autor: | E. G. Bade |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Time Factors
Transcription Genetic Polynucleotides Immunology RNA-dependent RNA polymerase Biology Nucleic Acid Denaturation Coliphages Microbiology Nucleic acid thermodynamics chemistry.chemical_compound Heavy strand Transcription (biology) Virology Centrifugation Density Gradient Escherichia coli RNA Messenger Lysogeny Polymerase Transcription bubble Nucleic Acid Hybridization RNA Molecular biology Microscopy Electron chemistry Insect Science DNA Viral Nucleic Acid Renaturation Bacterial Viruses biology.protein RNA Viral DNA |
| Zdroj: | Journal of Virology. 10:1205-1207 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.10.6.1205-1207.1972 |
| Popis: | The deoxyribonucleic acid (DNA) of bacteriophage Mu-1 can be separated into its complementary strands by poly(U,G) binding and equilibrium centrifugation. DNA-ribonucleic acid (RNA) hybridizations in liquid show that more than 98% of “early” phage-specific RNA and over 96% of “late” messenger species bind to the heavy [poly(U,G)-binding] strand of Mu-1 DNA. A small (1.45%) but significant amount of late RNA binds to the light strand. The significance of this RNA fraction is discussed in connection with the peculiar structure of denatured and reannealed Mu-1 DNA. |
| Databáze: | OpenAIRE |
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