ABC Transporters Required for Hexose Uptake by Clostridium phytofermentans
| Autor: | Magali Boutard, William Rostain, Alain Perret, Marcel Salanoubat, Christine Pelle, Andrew C. Tolonen, Tristan Cerisy, Henri-Pierre Fierobe, Alba Iglesias |
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| Přispěvatelé: | Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Génomique métabolique (UMR 8030), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE), Laboratoire de chimie bactérienne (LCB), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), ANR-16-CE05-0020,Phytocell,Développement des bactéries cellulolytiques Clostridium phytofermentans et Clostridium cellulolyticum comme biocatalyseurs pour la conversion de la biomasse végétale en alcools supérieurs(2016) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
ATP-binding cassette transporter
Microbiology Clostridia 03 medical and health sciences Clostridium Bacterial Proteins Hexose [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Sugar transporter Molecular Biology ComputingMilieux_MISCELLANEOUS 030304 developmental biology Hexoses 2. Zero hunger chemistry.chemical_classification 0303 health sciences biology 030306 microbiology food and beverages Biological Transport Clostridium phytofermentans biology.organism_classification chemistry Biochemistry ATP-Binding Cassette Transporters Anaerobic bacteria Bacteria Research Article |
| Zdroj: | Journal of Bacteriology Journal of Bacteriology, American Society for Microbiology, 2019, 201 (15), ⟨10.1128/JB.00241-19⟩ Journal of Bacteriology, 2019, 201 (15), ⟨10.1128/JB.00241-19⟩ |
| ISSN: | 0021-9193 1098-5530 |
| Popis: | The mechanisms by which bacteria uptake solutes across the cell membrane broadly impact their cellular energetics. Here, we use functional genomic, genetic, and biophysical approaches to reveal how Clostridium (Lachnoclostridium) phytofermentans, a model bacterium that ferments lignocellulosic biomass, uptakes plant hexoses using highly specific, nonredundant ATP-binding cassette (ABC) transporters. We analyze the transcription patterns of its 173 annotated sugar transporter genes to find those upregulated on specific carbon sources. Inactivation of these genes reveals that individual ABC transporters are required for uptake of hexoses and hexo-oligosaccharides and that distinct ABC transporters are used for oligosaccharides versus their constituent monomers. The thermodynamics of sugar binding shows that substrate specificity of these transporters is encoded by the extracellular solute-binding subunit. As sugars are not phosphorylated during ABC transport, we identify intracellular hexokinases based on in vitro activities. These mechanisms used by Clostridia to uptake plant hexoses are key to understanding soil and intestinal microbiomes and to engineer strains for industrial transformation of lignocellulose. IMPORTANCE Plant-fermenting Clostridia are anaerobic bacteria that recycle plant matter in soil and promote human health by fermenting dietary fiber in the intestine. Clostridia degrade plant biomass using extracellular enzymes and then uptake the liberated sugars for fermentation. The main sugars in plant biomass are hexoses, and here, we identify how hexoses are taken in to the cell by the model organism Clostridium phytofermentans. We show that this bacterium uptakes hexoses using a set of highly specific, nonredundant ABC transporters. Once in the cell, the hexoses are phosphorylated by intracellular hexokinases. This study provides insight into the functioning of abundant members of soil and intestinal microbiomes and identifies gene targets to engineer strains for industrial lignocellulosic fermentation. |
| Databáze: | OpenAIRE |
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