A Lipid Bilayer Formed on a Hydrogel Bead for Single Ion Channel Recordings
| Autor: | Daiki Yamamoto, Toru Ide, Akinobu Matsumoto, Tohru Hayakawa, Shintaro Mise, Mami Asakura, Minako Hirano |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
BK channel
Materials science single-channel recording lcsh:Mechanical engineering and machinery KcsA potassium channel 02 engineering and technology Article 03 medical and health sciences chemistry.chemical_compound lcsh:TJ1-1570 Electrical and Electronic Engineering Lipid bilayer Ion channel Ion transporter 030304 developmental biology 0303 health sciences Alamethicin biology Mechanical Engineering 021001 nanoscience & nanotechnology Potassium channel lipid bilayer chemistry Control and Systems Engineering ion channel Gramicidin biology.protein Biophysics 0210 nano-technology |
| Zdroj: | Micromachines Volume 11 Issue 12 Micromachines, Vol 11, Iss 1070, p 1070 (2020) |
| ISSN: | 2072-666X |
| Popis: | Ion channel proteins play important roles in various cell functions, making them attractive drug targets. Artificial lipid bilayer recording is a technique used to measure the ion transport activities of channel proteins with high sensitivity and accuracy. However, the measurement efficiency is low. In order to improve the efficiency, we developed a method that allows us to form bilayers on a hydrogel bead and record channel currents promptly. We tested our system by measuring the activities of various types of channels, including gramicidin, alamethicin, &alpha hemolysin, a voltage-dependent anion channel 1 (VDAC1), a voltage- and calcium-activated large conductance potassium channel (BK channel), and a potassium channel from Streptomyces lividans (KcsA channel). We confirmed the ability for enhanced measurement efficiency and measurement system miniaturizion. |
| Databáze: | OpenAIRE |
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