Identification of clinical isolates of α-hemolytic streptococci by 16S rRNA gene sequencing, matrix-assisted laser desorption ionization-time of flight mass spectrometry using MALDI Biotyper, and conventional phenotypic methods: a comparison

Autor:	Howard F. Jenkinson, Angharad P. Davies, Khalid El-Bouri, Michelle Reid, Dietrich Mack, Stephen J. Hadfield, Ann M. Lewis, Nidhika Berry, Stuart Johnston, Jane Mikhail, Llinos G. Harris
Rok vydání:	2012
Předmět:	Microbiology (medical) DNA Bacterial Sequence analysis Matrix assisted laser desorption ionization time of flight Biology Mass spectrometry medicine.disease_cause DNA Ribosomal Microbiology fluids and secretions Species level RNA Ribosomal 16S Streptococcal Infections medicine Humans Streptococcus Bacteriology Sequence Analysis DNA Ribosomal RNA 16S ribosomal RNA bacterial infections and mycoses equipment and supplies Molecular biology Bacterial Typing Techniques Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization 16s rrna gene sequencing DNA Intergenic
Zdroj:	Journal of clinical microbiology. 50(12)
ISSN:	1098-660X
Popis:	Fifty-six α-hemolytic streptococcal isolates were identified using MALDI Biotyper MS (Bruker Daltonics), API 20 Strep (bioMérieux), and BD Phoenix (Becton, Dickinson). The gold standard for identification was 16S rRNA gene sequence analysis with 16S-23S rRNA intergenic spacer sequencing. The following percentages of isolates were correctly identified to the species level: MALDI Biotyper, 46%; BD Phoenix, 35%; and API 20 Strep, 26%.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73bfc23c6b1cd67e63d442a7d3687f50 https://pubmed.ncbi.nlm.nih.gov/22993176 Zobrazit plný text záznamu