Population dynamics of methicillin-susceptible and -resistant Staphylococcus aureus in remote communities

Autor: Warren B. Grubb, F. Moss, Michael Gracey, Geoffrey W. Coombs, John W. Pearman, Keryn J. Christiansen, Frances G. O'Brien
Rok vydání: 2009
Předmět:
DNA
Bacterial
Methicillin-Resistant Staphylococcus aureus
Rural Population
Microbiology (medical)
Bacterial Toxins
Population
Exotoxins
Microbial Sensitivity Tests
Biology
Staphylococcal infections
medicine.disease_cause
Polymerase Chain Reaction
Microbiology
Bacterial Proteins
Leukocidins
medicine
Cluster Analysis
Humans
Pharmacology (medical)
Typing
education
Original Research
Pharmacology
Molecular Epidemiology
education.field_of_study
Molecular epidemiology
community methicillin-resistant Staphylococcus aureus
population structure
Sequence Analysis
DNA
Western Australia
Staphylococcal Infections
biochemical phenomena
metabolism
and nutrition
S. aureus
colonization
bacterial infections and mycoses
medicine.disease
DNA Fingerprinting
Methicillin-resistant Staphylococcus aureus
Bacterial Typing Techniques
Electrophoresis
Gel
Pulsed-Field
Community-Acquired Infections
Infectious Diseases
Staphylococcus aureus
Carrier State
Trans-Activators
Multilocus sequence typing
Methicillin Susceptible Staphylococcus Aureus
Zdroj: Journal of Antimicrobial Chemotherapy
ISSN: 1460-2091
0305-7453
DOI: 10.1093/jac/dkp285
Popis: Objectives: Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) was first reported in remote regions of Western Australia (WA) in 1992 and is now the predominant MRSA isolated in the State. To gain insights into the emergence of CA-MRSA, 2146 people living in 11 remote WA communities were screened for colonization with S. aureus. Methods: Antibiogram analysis, contour-clamped homogeneous electric field electrophoresis, multilocus sequence typing, Panton-Valentine leucocidin determinant detection and accessory genetic regulator typing were performed to characterize the isolates. MRSA was further characterized by staphylococcal cassette chromosome mec typing. Results: The S. aureus population consisted of 13 clonal complexes and two Singleton lineages together with 56 sporadic isolates. Five lineages contained MRSA; however, these were not the predominant methicillin-susceptible S. aureus (MSSA) lineages. There was greater diversity amongst the MSSA while the MRSA appeared to have emerged clonally following acquisition of the staphylococcal cassette chromosome mec. Three MRSA lineages were considered to have been endemic in the communities and have subsequently become predominant lineages of CA-MRSA in the wider WA community. People colonized with MSSA tended to harbour clones of a different genetic lineage at each anatomical site while people colonized with MRSA tended to harbour clones of the same lineage at each site. Overall, the isolates were resistant to few antimicrobials. Conclusions: Although the evidence suggests that in WA CA-MRSA strains arose in remote communities and have now disseminated into the wider community, there is no evidence that they arose from the predominant MSSA clones in these communities.
Databáze: OpenAIRE
Externí odkaz: