Overexpression of ING3 is associated with attenuation of migration and invasion in breast cancer

Autor: Mingjian Tan, Dequan Liu, Maohua Wang, Huimeng Li, Hongwan Li, Shicong Tang, Rui Liu, Wenlin Chen, Kai Zheng, Hengyu Zhang, Rong Guo, Ke Wang, Xin Tan, Yiyin Tang
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Experimental and Therapeutic Medicine
ISSN: 1792-1015
1792-0981
Popis: Inhibitor of growth 3 (ING3) has been identified as a potential cancer drug target, but little is known about its role in breast cancer. Thus, the present study aimed to investigate ING3 expression in breast cancer, its clinical value, and how ING3 influences the migration and invasion of breast cancer cells. The Cancer Genome Atlas and UALCAN databases were used to analyze ING3 expression in cancer tissues and normal tissues. Survival analysis was performed using the UALCAN, UCSC Xena and KM-plot databases. In addition, reverse transcription-quantitative PCR and western blot analyses were performed to detect ING3 mRNA and protein expression levels. ING3 was overexpressed via lentiviral vector transfection, while the Transwell and wound healing assays were performed to assess the cell migratory and invasive abilities. Protein interaction and pathway analyses were performed using the GeneMANIA and Kyoto Encyclopedia of Genes and Genomes databases, respectively. The results demonstrated that ING3 expression was significantly lower in cancer tissues compared with normal tissues (P
Databáze: OpenAIRE
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