Immunoglobulins from sera of antiphospholipid syndrome patients are internalized in the HTR-8/SVneo cell line and cytotrophoblast in culture
| Autor: | Ljiljana Vićovac, Srđa Janković, Milica Jovanović Krivokuća, Tamara Abu Rabi, Milan Marković, Svetlana Vrzić-Petronijević, Ivana Stefanoska |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Immunology Immunocytochemistry Cell Line Flow cytometry Andrology 03 medical and health sciences 0302 clinical medicine Syncytiotrophoblast Pregnancy immune system diseases Antiphospholipid syndrome Humans Immunology and Allergy Medicine neoplasms 030219 obstetrics & reproductive medicine Cytotrophoblast medicine.diagnostic_test biology business.industry Trophoblast Hematology Antiphospholipid Syndrome medicine.disease Trophoblasts 3. Good health Pregnancy Complications Pregnancy Trimester First 030104 developmental biology medicine.anatomical_structure Cell culture embryonic structures Antibodies Antiphospholipid biology.protein Female Chorionic Villi Antibody business |
| Zdroj: | Immunobiology. 223:544-548 |
| ISSN: | 0171-2985 |
| Popis: | Women with antiphospholipid syndrome (APS) experience pregnancy complications mostly due to impaired trophoblast cell functions. Antiphospholipid antibodies (aPL) affect extravillous trophoblast in vivo and in culture, but the mechanisms are still poorly understood. Previously, syncytiotrophoblast was shown to bind and internalize aPL, which was not replicated for extravillous cytotrophoblast in short term culture. Here, aPL binding and time dependent internalization was demonstrated with exposure to aPL in the extravillous cell line HTR-8/SVneo and isolated first trimester of pregnancy cytotrophoblast (CT) using immunocytochemistry and flow cytometry. Intracellular aPL were detectable from 2 h of culture, reaching 30.7 ± 3.1% (p < 0.001) positive cells in CT and 24.8 ± 7% (p < 0.01) in HTR-8/SVneo cells at 24 h and 33 ± 4.2% (p < 0.01) at 48 h. The data presented show that extravillous trophoblast cells internalize aPL in a time-dependent manner significantly more than control immunoglobulins after 24 h of exposure. |
| Databáze: | OpenAIRE |
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