Structure-Function Insights of Jaburetox and Soyuretox: Novel Intrinsically Disordered Polypeptides Derived from Plant Ureases
| Autor: | Anne H.S. Martinelli, Fernanda Cortez Lopes, Matheus V. Coste Grahl, Célia R. Carlini, Leonardo L. Fruttero |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
biopesticides Urease INSECTICIDAL ACTIVITY Pharmaceutical Science Review Intrinsically disordered proteins ANTIFUNGAL ACTIVITY Analytical Chemistry insecticidal activity purl.org/becyt/ford/1 [https] lcsh:QD241-441 03 medical and health sciences Structure-Activity Relationship MECHANISM OF ACTION lcsh:Organic chemistry Drug Discovery Recombinant peptide Amino Acid Sequence transgenic crops Physical and Theoretical Chemistry Pesticides purl.org/becyt/ford/1.6 [https] Protein secondary structure Plant Proteins 030102 biochemistry & molecular biology biology Chemistry TRANSGENIC CROPS Organic Chemistry Structure function antifungal activity biology.organism_classification BIOPESTICIDES Intrinsically Disordered Proteins 030104 developmental biology Biochemistry Chemistry (miscellaneous) Canavalia ensiformis Glycine biology.protein Molecular Medicine Fish Peptides mechanism of action |
| Zdroj: | Molecules, Vol 25, Iss 5338, p 5338 (2020) Molecules CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 1420-3049 |
| Popis: | Intrinsically disordered proteins (IDPs) and intrinsically disordered regions (IDRs) do not have a stable 3D structure but still have important biological activities. Jaburetox is a recombinant peptide derived from the jack bean (Canavalia ensiformis) urease and presents entomotoxic and antimicrobial actions. The structure of Jaburetox was elucidated using nuclear magnetic resonance which reveals it is an IDP with small amounts of secondary structure. Different approaches have demonstrated that Jaburetox acquires certain folding upon interaction with lipid membranes, a characteristic commonly found in other IDPs and usually important for their biological functions. Soyuretox, a recombinant peptide derived from the soybean (Glycine max) ubiquitous urease and homologous to Jaburetox, was also characterized for its biological activities and structural properties. Soyuretox is also an IDP, presenting more secondary structure in comparison with Jaburetox and similar entomotoxic and fungitoxic effects. Moreover, Soyuretox was found to be nontoxic to zebra fish, while Jaburetox was innocuous to mice and rats. This profile of toxicity affecting detrimental species without damaging mammals or the environment qualified them to be used in biotechnological applications. Both peptides were employed to develop transgenic crops and these plants were active against insects and nematodes, unveiling their immense potentiality for field applications. Fil: Grahl, Matheus V. Coste. Pontificia Universidade Católica do Rio Grande do Sul; Brasil Fil: Lopes, Fernanda Cortez. Universidade Federal do Rio Grande do Sul; Brasil Fil: Martinelli, Anne H. Souza. Universidade Federal do Rio Grande do Sul; Brasil Fil: Carlini, Célia Regina R. S.. Pontificia Universidade Católica do Rio Grande do Sul; Brasil Fil: Fruttero, Leonardo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina |
| Databáze: | OpenAIRE |
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