Molecular identification and phylogenetic characterization of influenza A virus at a wildlife–livestock interface in Mexico
| Autor: | Ivan Sanchez-Betancourt, Heliot Zarza, René Segura-Velázquez, Jessica Mateus-Anzola, Liliana Gaytan-Cruz, Cecilia Montoya-Carrillo, Rafael Ojeda-Flores |
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| Rok vydání: | 2020 |
| Předmět: |
Livestock
Marsh Swine 040301 veterinary sciences animal diseases Wildlife Hemagglutinin (influenza) Zoology Animals Wild medicine.disease_cause law.invention 0403 veterinary science 03 medical and health sciences Influenza A Virus H1N1 Subtype law Waterfowl Influenza A virus medicine Animals Mexico Phylogeny 030304 developmental biology Swine Diseases Mexican duck 0303 health sciences geography geography.geographical_feature_category General Veterinary General Immunology and Microbiology biology Phylogenetic tree business.industry Influenza A Virus H3N2 Subtype Outbreak 04 agricultural and veterinary sciences General Medicine biology.organism_classification United States Transmission (mechanics) Influenza in Birds biology.protein Influenza A Virus H5N2 Subtype business |
| Zdroj: | Transboundary and Emerging Diseases. 68:3563-3573 |
| ISSN: | 1865-1682 1865-1674 |
| Popis: | Influenza A virus (IAV) outbreaks constitute a constant threat to public health and pose a remarkable impact on socio-economic systems worldwide. Interactions between wild and domestic birds, humans, and swine can lead to spillover events. Backyard livestock systems in proximity to wetlands represent a high-risk area for viral spread. However, some gaps remain in our knowledge of IAV transmission at the wildlife – livestock interface in Mexico. Hence, the study aimed at molecular identification and phylogenetic characterization of IAV in the wild duck – backyard livestock interface at a wetland of Mexico. A total of 875 animals were tested by real-time RT-PCR (qRT-PCR). We detected IAV in 3.68% of the wild ducks sampled during the winter season 2016 – 2017. Nonetheless, the samples obtained from backyard poultry and swine tested negative. The highest IAV frequency (11.10%) was found in the Mexican duck (Anas diazi). Subtypes H1N1, H3N2, and H5N2 were detected. Phylogenetic analysis of influenza viruses isolated from wild ducks of the Lerma marshes revealed that hemagglutinin (HA) gene sequences were related to waterfowl, swine, and poultry IAV strains previously isolated in the United States and Mexico. In conclusion, the co-circulation of three IAV subtypes in wild ducks close to backyard farms in Mexico, as well as, the local identification of HA gene sequences genetically related to Mexican livestock IAV strains and also to North American waterfowl IAV strains, highlight the importance of the Lerma marshes for influenza surveillance given the close interaction among wild birds, poultry, pigs, and humans. |
| Databáze: | OpenAIRE |
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