Palmitoylation of the Bovine Foamy Virus Envelope Glycoprotein Is Required for Viral Replication

Autor: Junshi Zhang, Zhaohuan Wang, Juan Tan, Keli Chai, Wentao Qiao, Yali Xu
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Viruses
Volume 13
Issue 1
Viruses, Vol 13, Iss 31, p 31 (2021)
ISSN: 1999-4915
DOI: 10.3390/v13010031
Popis: Membrane proteins of enveloped viruses have been reported to undergo palmitoylation, a post-translational modification often having a critical role in the function of these viral proteins and hence viral replication. In this study, we report that the foamy virus (FV) envelope (Env) glycoprotein is palmitoylated. Specifically, we found that bovine foamy virus (BFV) Env (BEnv) is palmitoylated at amino acid positions C58 and C59 by BDHHC3 and BDHHC20 in a DHHC motif-dependent manner. In addition, mutations C58S and C58/59S significantly decrease cell surface expression of BEnv, subviral particle (SVP) egress, and its membrane fusion activity, thus ultimately inhibiting BFV replication. The C59S mutation exerts a minor effect in this regard. Taken together, these data demonstrate that the function of BEnv in the context of BFV replication is under the regulation of palmitoylation.
Databáze: OpenAIRE
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