Impact of Environmental and Genetic Factors on Biofilm Formation by the Probiotic Strain Lactobacillus rhamnosus GG
| Autor: | Tine Verhoeven, Sigrid C. J. De Keersmaecker, Jos Vanderleyden, Mónica Perea Vélez, Sarah Lebeer |
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| Rok vydání: | 2007 |
| Předmět: |
Lipopolysaccharides
Lactobacillus casei Public Health Microbiology Applied Microbiology and Biotechnology Bacterial Adhesion law.invention Microbiology Probiotic fluids and secretions Bacterial Proteins Lactobacillus rhamnosus law Lactobacillus Ecology biology Lacticaseibacillus rhamnosus Probiotics Polysaccharides Bacterial Mucin Biofilm food and beverages Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition biology.organism_classification Mucus Teichoic Acids Carbon-Sulfur Lyases Genes Bacterial Biofilms Lipoteichoic acid Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 73:6768-6775 |
| ISSN: | 1098-5336 0099-2240 |
| Popis: | Lactobacillus rhamnosus GG (ATCC 53103) is one of the clinically best-studied probiotic organisms. Moreover, L. rhamnosus GG displays very good in vitro adherence to epithelial cells and mucus. Here, we report that L. rhamnosus GG is able to form biofilms on abiotic surfaces, in contrast to other strains of the Lactobacillus casei group tested under the same conditions. Microtiter plate biofilm assays indicated that in vitro biofilm formation by L. rhamnosus GG is strongly modulated by culture medium factors and conditions related to the gastrointestinal environment, including low pH; high osmolarity; and the presence of bile, mucins, and nondigestible polysaccharides. Additionally, phenotypic analysis of mutants affected in exopolysaccharides ( wzb ), lipoteichoic acid ( dltD ), and central metabolism ( luxS ) showed their relative importance in biofilm formation by L. rhamnosus GG. |
| Databáze: | OpenAIRE |
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