Insights from the shotgun-based proteomics analysis of Biomphalaria glabrata

Autor:	Mofolusho O. Falade, Castro-Borges William, Benson Otarigho
Rok vydání:	2021
Předmět:	hemolymph animal structures biology Bioinformatics fungi Schistosomiasis Shotgun General Medicine Snail medicine.disease Proteomics biology.organism_classification Biomphalaria glabrata Biochemistry biology.animal parasitic diseases Proteome Hemolymph Shotgun proteomics medicine Research Article
Zdroj:	Bioinformation
ISSN:	0973-2063 0973-8894
DOI:	10.6026/97320630017266
Popis:	Biomphalaria glabrata is an important host in the transmission of human schistosomiasis in the Caribbean and South America. Therefore, it is of interest to analyse the proteome data of Biomphalaria glabrata hemolymph to identify immunity related proteins in host-pathogen relationship. We used shotgun proteomic and bioinformatic analyses of the non-depleted and depleted [0.5 and 0.75% Trifluoroacetic acid (TFA) depletion] hemolymph of B. glabrata (LE strain). Analysis showed 148 proteins from the hemolymph. 148 were obtained from the 0.5% TFA-depleted sample. 62 proteins follow this from the 0.75% TFA-depleted sample. However, only 59 were found from non-depleted hemolymph. A number of proteins were identified from the hemolymph of this schistosomiasis snail vector linked to immunity related functions. This provides insights to the understanding of schistosome-snail interaction.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73b41c6ae955f9dd3871f530d8532c7a https://doi.org/10.6026/97320630017266 Zobrazit plný text záznamu