Nasal obstruction during the growth period modulates the Wnt/β-catenin pathway and brain-derived neurotrophic factor production in association with tyrosine kinase receptor B mRNA reduction in mouse hippocampus
| Autor: | Takuya Ogawa, Akiyo Fujita, Chiho Kato, Phyo Thura Aung, Hidemasa Okihara, Satoshi Kokai, Hideyuki Ishidori, Yasunori Abe, Takashi Ono |
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| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty Hippocampus Tropomyosin receptor kinase B Hippocampal formation Biology Mice Neurotrophic factors Internal medicine Wnt3A Protein medicine Animals Receptor trkB RNA Messenger beta Catenin Brain-derived neurotrophic factor Mice Inbred BALB C General Neuroscience Brain-Derived Neurotrophic Factor Wnt signaling pathway Receptor Protein-Tyrosine Kinases Endocrinology Catenin Nasal Obstruction |
| Zdroj: | The European journal of neuroscience. 55(1) |
| ISSN: | 1460-9568 |
| Popis: | There is accumulating evidence that nasal obstruction induces high-level brain dysfunction, including memory and learning deficits. We previously demonstrated that unilateral nasal obstruction (UNO) during the growth period increases the expression of brain-derived neurotrophic factor (BDNF). The expression of BDNF is regulated by the Wnt/β-Catenin pathway, which is linked to neuronal differentiation, proliferation, and maintenance. However, little is known about whether Wnt3a protein expression could be an index for modulations analyses in the Wnt/β-Catenin pathway caused by UNO during the growth period. This study aimed to investigate the effects of UNO during the growth period on the Wnt/β-Catenin pathway in the hippocampus using combined behavioral, biochemical, and histological approaches. Male BALB/C mice were randomly divided into the control (CONT; n=6) and experimental (UNO; n=6) groups. Blood oxygen saturation (SpO2 ) levels were measured, and a passive avoidance test was performed in mice aged 15 weeks. Brain tissues were subjected to immunohistochemistry, real-time reverse transcription-polymerase chain reaction, and western blot analysis. Compared with control mice, UNO mice had lower SpO2 levels and exhibited memory/learning impairments during behavioral testing. Moreover, Wnt3a protein, BDNF mRNA, and tyrosine kinase receptor B (TrkB) mRNA expression levels were significantly lower in the hippocampus in the UNO group than in the CONT group. Our findings suggested that UNO during the growth period appeared to modulate the hippocampal Wnt/β-catenin pathway and BDNF production in association with TrkB mRNA reduction, thereby resulting in memory and learning impairments. |
| Databáze: | OpenAIRE |
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