Expression of hPNAS-4 Radiosensitizes Lewis Lung Cancer
Autor: | Qian Deng, Yu Fan, Hong Zhu, Zhiping Li, He Yinbo, Huashan Shi, Jianghong Xiao, Jianshuang Zeng, Zhu Yuan, Zi Wang, Lei Li, Hui Zeng |
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Rok vydání: | 2012 |
Předmět: |
Cancer Research
Pathology medicine.medical_specialty Lung Neoplasms Genetic enhancement medicine.medical_treatment Apoptosis Transfection Carcinoma Lewis Lung Mice Random Allocation Western blot Cell Line Tumor Carbon-Nitrogen Lyases In Situ Nick-End Labeling Animals Humans Medicine Radiology Nuclear Medicine and imaging RNA Small Interfering Tumor Stem Cell Assay Radiation TUNEL assay medicine.diagnostic_test business.industry Flow Cytometry Neoplasm Proteins Tumor Burden Mice Inbred C57BL Radiation therapy Oncology Cell culture Cancer research Female Apoptosis Regulatory Proteins business |
Zdroj: | International Journal of Radiation Oncology*Biology*Physics. 84:e533-e540 |
ISSN: | 0360-3016 |
Popis: | Purpose This study aimed to transfer the hPNAS-4 gene, a novel apoptosis-related human gene, into Lewis lung cancer (LL2) and observe its radiosensitive effect on radiation therapy in vitro and in vivo. Methods and Materials The hPNAS-4 gene was transfected into LL2 cells, and its expression was detected via western blot. Colony formation assay and flow cytometry were used to detect the growth and apoptosis of cells treated with irradiation/PNAS-4 in vitro. The hPNAS-4 gene was transferred into LL2-bearing mice through tail vein injection of the liposome/gene complex. The tumor volumes were recorded after radiation therapy. Proliferating cell nuclear antigen (PCNA) immunohistochemistry staining and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay were performed to detect the tumor cell growth and apoptosis in vivo. Results The hPNAS-4 gene was successfully transferred into LL2 cells and tumor tissue, and its overexpressions were confirmed via western blot analysis. Compared with the control, empty plasmid, hPNAS-4 , radiation, and empty plasmid plus radiation groups, the hPNAS-4 plus radiation group more significantly inhibited growth and enhanced apoptosis of LL2 cells in vitro and in vivo ( P Conclusions The hPNAS-4 gene was successfully transferred into LL2 cells and tumor tissue and was expressed in both LL2 cell and tumor tissue. The hPNAS-4 gene therapy significantly enhanced growth inhibition and apoptosis of LL2 tumor cells by radiation therapy in vitro and in vivo. Therefore, it may be a potential radiosensitive treatment of radiation therapy for lung cancer. |
Databáze: | OpenAIRE |
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