In vitro screening of radioprotective properties in the novel glucosylated flavonoids
| Autor: | Takamitsu A. Kato, Yasushi Aizawa, Victoria A. Salinas, Shigeaki Sunada, Taisuke Inoue, Mitsuru Uesaka, Allison J. Allum, Alexis H. Haskins, Jeremy S. Haskins, Hao Yu, Cathy Su |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Naringenin Glycosylation Radioprotective Agent Flavonoid Drug Evaluation Preclinical Radiation-Protective Agents Biology Antioxidants 03 medical and health sciences Hesperidin chemistry.chemical_compound Rutin 0302 clinical medicine Picrates Nephelometry and Turbidimetry Genetics DNA Breaks Double-Stranded heterocyclic compounds Electrophoresis Agar Gel Flavonoids chemistry.chemical_classification Biphenyl Compounds fungi Hesperetin food and beverages Free Radical Scavengers General Medicine Molecular biology carbohydrates (lipids) 030104 developmental biology Biochemistry chemistry 030220 oncology & carcinogenesis Isoquercetin Quercetin |
| Zdroj: | International Journal of Molecular Medicine. 38:1525-1530 |
| ISSN: | 1791-244X 1107-3756 |
| Popis: | Novel glucosyl flavonoids are developed by the addition of glucose to naturally occurring flavonoids. Flavonoids are known antioxidants that possess radioprotective properties. In order to investigate the radioprotective properties of novel glucosyl flavonoids, in vitro DNA double-strand breaks (DSBs) analysis was carried out. In the present study, Quercetin, Naringenin, and Hesperetin groups of flavonoids included in the natural and novel glucosyl 13 flavonoids were investigated. Flavonoids were mixed with Lambda DNA, and subsequently exposed to gamma‑rays. Furthermore, DNA DSB yields were visualized by gel electrophoresis. Quercetin derivatives displayed reduced DNA DSB formation at 10 µM. At a high concentration, the majority of flavonoids displayed radioprotective properties as a reduction of DSB yields. Suppression of DSB formation was confirmed via the molecular combing assay for Quercetin, and three monoglucosyl flavonoids. Glucosylation showed positive effects for radioprotection and monoglucosyl-Rutin showed superior radioprotective properties when compared to monoglucosyl-Naringin and Hesperidin. In addition, Quercetin derivatives had greater total antioxidant capacities and DPPH radical scavenging ability than other flavonoid groups. Since Quercetin, Isoquercetin, and Rutin display poor water solubility, monoglucosyl-Rutin, maltooligosyl-Isoquercetin, and maltooligosyl-Rutin may be better radioprotective agents and easily bioavailable with increased water solubility. |
| Databáze: | OpenAIRE |
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