Elicitation of Th1/Th2 related responses in mice by chitosan nanoparticles loaded with Brucella abortus malate dehydrogenase, outer membrane proteins 10 and 19

Autor: Han Sang Yoo, Chong-Su Cho, Soojin Shim, Young Bin Im, Suk Kim, Hyun-Eui Park, Sang Hee Soh
Jazyk: angličtina
Rok vydání: 2020
Předmět:
animal diseases
Brucella Vaccine
Brucella abortus
law.invention
Mice
Other systems of medicine
Immunogenicity
Vaccine
Mucosal immunity
law
Malate Dehydrogenase
Pathogen
0303 health sciences
Mice
Inbred BALB C
biology
General Medicine
Antibodies
Bacterial
Recombinant Proteins
QR1-502
Infectious Diseases
Recombinant DNA
Cytokines
Female
Bacterial outer membrane
Immunogenic antigens
Bacterial Outer Membrane Proteins
Microbiology (medical)
chemical and pharmacologic phenomena
Brucella
Malate dehydrogenase
Subunit vaccine
Microbiology
Brucellosis
03 medical and health sciences
Interferon-gamma
Immune system
Th2 Cells
Antigen
Animals
Antigen cocktail
030304 developmental biology
Chitosan
030306 microbiology
Th1 Cells
biology.organism_classification
Chitosan nanoparticle
Immunoglobulin G
Nanoparticles
bacteria
Nasal administration
Immunization
RZ201-999
Zdroj: International Journal of Medical Microbiology, Vol 310, Iss 1, Pp-(2020)
ISSN: 1438-4221
Popis: Brucella spp. is the causative agent of brucellosis, one of the worldwide diseases. The pathogen infects humans and animals mainly through the digestive or respiratory tract. Therefore, induction of mucosal immunity is required as the first line of defense. In this study, three Brucella abortus recombinant proteins, malate dehydrogenase (rMdh), outer membrane proteins (rOmp) 10 and 19 were loaded in mucoadhesive chitosan nanoparticles (CNs) and induction of mucosal and systemic immunity were investigated after intranasal immunization of BALB/c mice. These antigens were also coimmunized as cocktail (rCocktail) to evaluate multiple antigen specific vaccine candidates. At 6-weeks post-immunization (wpi), antigen specific total IgG was increased in all of the immunized groups, predominantly IgG1. In addition, spleenocyte from rMdh-, rOmp19-, and rCocktail-immunized groups significantly produced IFN-γ and IL-4 suggesting the induction of a mixed Th1-Th2 response. For mucosal immunity, anti-Mdh IgA from nasal washes and fecal excretions, and anti-Omps IgA from sera, nasal washes, genital secretions and fecal excretions were significantly increased in single antigen immunized groups. In the rCocktail-immunized group, anti-Mdh IgA were significantly increased while anti-Omps IgA was not. Collectively, this study indicates that comprise of B. abortus antigen-loaded CNs elicited the antigen-specific IgA with a Th2-polarized immune responses and combination of the highly immunogenic antigens elicited IgG specific to each type of antigen.
Databáze: OpenAIRE
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